A Raw Spore Cytophaga Bacteria Separation And Purification, Identification And Degradation Process Of Cellulose Filter Paper,

A strain of cellulolytic bacterium was isolated from soil by filter paper plate and cellulose fibre double-plate. The properties of morphology and physiology of this strain was studied. According to Bergey's Manual of Determinative Bacteriology, the strain was identified as a new strain of the Sporocytophaga.Sporocytophaga is a kind of bacteria that can glide on the surface of solid medium .This strain can decompose the cellulose strongly.lt can grow on the surface of cotton and fiter paper, and produce large amount of extracellular polysacchrides during the cellulose degradation. The sporocytophag can only produce a low extracellular carboxymethyl-cellulase(CMC) activity and no other extracellular cellulase activities .So the Sporocytophaga has a special mechanism of cellulose degradation.In this work, the process that a strain of Sporocytophaga degraded the filter paper fibre was studied by means of scan electronmicroscopy.The filter paper cellulose degraded rule and the morphology change on different phase were analysed. The result showed that this strain have a strong ability to decompose the filter paper cellulose. In the process of degrading fibre ,the strain adhere tightly to the fibre through its bacilliform cell. The bacilliform cell penetrate into interior of the fibre to degrade the cellulose strongly and produced a mass of sticky polysaccharides. After cultured 48 hours ,the bacilliform cell's surface of Sporocytophaga have a great change .At this stage the bacilliform produce a lot of sticky polysaccharides.These sticky polysaccharides associated with the sites where the filter paper was decomposed intensively and form thorns on the surface of the bacillium.At the same time ,the filter-paper weight loss is the greatest and decomposing rate is the fastest ,so we think that the sticky polysaccharides are produced during the cellulose degradation. At theanaphase of cultured, the bacteria exsist as a cycloidal dormancy body-sporocyst,and the decomposing rate of filter-paper began to decrease.We know the sporocyst is a form that sporocytophaga genus appear in a ill circumstance,so we think the sporocyst didn't have the cellulose decomposing activity.After cultered 96 hours, we can found many sporocysts and filter-paper slices in the fermentable liquid. This strain can decompose the filter paper fibre entirely after cultured 6-7 days.