Electrochemical Method For Preparation Of Dna Chip

Linker molecules, the polymerization of 3-[2, 5, 8, 11-tetraoxa-tridecyl-13-ol]- pyrrole were designed and synthesized according to the requirement of the electropolymerization. The electropolymerization of 3-pyrrole derivative with alkaline medium at platinum disk electrode have been studied by cyclic voltammetry and chronoamprometry. The polypyrrole membrane was characterized with Scanning Electro Microscopy (SEM), Energy Dispersive X-ray Analysis (EDX). The results show that the pyrrole-derivative was successfully electropolymerized on the surface of the electrode. The data estimated by EDX results revealed the density of the pyrrole-derivative on the electrode was about several nmol per square milimeter.The 3-pyrrole-DNA also was successfully deposited on the electrode surface by electropolymerization because the EDX characterization of polymer shows that the phosphor element was appeared. Finally, the hybridization on the 3-pyrrole-DNA polymer modified electrode was detected with methylene blue (MB) as a hybridization indicator, thus the function of 3-pyrrole-DNA modified electrode was qualitatively analyzed. A micro-array electrode (MAE) of gold was designed and factored by microelectronic machining techniques. The electrochemical behaviors of the MAE are characterized with cyclic voltammetry. From the results of the experiment, we can see that simultaneity electrochemical polymerization of 3-pyrrole-DNA and 3-[2, 5, 8, 11-tetraoxa-tridecyl-13-ol]- pyrrole was a new promising method for preparing DNA chips because this method had such following advantages: First, the immobilization procedure can be controlled easily. Second, with the progress of microelectronics, the miniaturization and integraterization of polypyrrole-based DNA chip can be achieved in the future; Third, using an electrochemical active compound like the MB, the hybridization detection will be performed more easily.