Fungus Fruiting Bodies Of Hair Handle Gold Wire Stipe Cell Wall Elongation

We identified a Flammulina velutipes stipe cell walls extension protein from β-glucuronidase with potent ability to induce extension of heat-inactivated stipe cell walls. It could increase rate of extension at very short times when it was added in the room of extensmeter. It was purified and separated by gel filtration, ion-exchange chromagraphy and HPLC Synchropak S300 ion-exchange chromagraphy. By SDS-PAGE, the molecular weight of protein was estimated as 21KD. It has no hydrolytic enzyme activities against chitin, and did not exhibit β-glucananase and Xyloglucanase, Glucomananase activities.Through analysis of using mass spectrum of LCQ-DECA-XP at Shanghai Institues for Biological Sciences, we had not found matching fragments. In Haward University, the method of Combinent Edman and LS/MS/MS was adopted, we gained two fragments of EFHWAVPK and MFSNDVLAR, but also did not find the completely matching fragments and conferred it as a new unknown protein.We also find a 33KD β-1,3-glucanase. It has strong hydrolytic enzyme activity against barley β-glucan, but did not exhibit cellulose activities. The optimal temperature and pH of this enzyme were 37℃ and 4. 5.Respectively,the β-1, 3-glucanase was more stable at low pH no more than pH 8.0, and was relatively stable below 50 ℃.Through analysis using mass spectrum of LCQ-DECA-XP,we did not find the completely matching protein and conferred it as a new β -1,3-glucanase.