Promoter Characterization And Transcriptional Regulation Of Human Kctd10 Gene

Human potassium channel tetramerisation domain-containing 10 gene (KCTD10) belongs to polymerase delta-interacting protein 1 (PDIP1) gene family. Recent studies demonstrated that mouse KCTD10 can interact with PCNA and the small subunit of polymeraseδ(p50), and may play roles in DNA repair, DNA replication, cell apoptosis and cell cycle control. In this study, we have isolated and characterized human KCTD10 gene promoter containing a 639 bp fragment of the 5' franking region (-609/+30). The transcription start site (TSS) was determined by primer extension assay to be A, 63 bp upstream of the translation start codon. The promoter region contains no TATA box or CCAAT box, instead, a CpG island was observed close to TSS. Deletion mutagenesis showed that the region from -108 to +30 was indispensable to the basal promoter activity. Moreover, many potential transcription factors were found in the proximal promoter region .We also confirmed that both Sp1 and AP-2 can bind to the proximal promoter region and modulated the promoter activity, with Spl activated, while AP-2 suppressed the expression of human KCTDlO.