The Enzymatic Properties Of Soybean Peroxidase And Applied Research

Soybean peroxidase (SBP) obtained from the soybean coats belongs to class III of the plant peroxidase superfamily(EC1.11.1.7), similar to horseradish peroxidase (HRP). SBP is very stable at high temperature, extremes of pH, and in organic solvent. At the same time, it is highly reactive towards both organic and inorganic substrates, similar to horseradish peroxidase. SBP has a wide range of potential applications,and its structure is of particular interest for engineering purposes and as a model for stable heme peroxidases. SBP has been found to be a very effective of biocatalyst and biosensor which has been used in wastewater treatment and phenolic resin synthesis.SBP is a highly heterogeneous glycoprotein with MS determined masses from 39 to 41 kDa. The mature protein consists of 306 residues starting with pyrrolidone carboxylic acid. SBP is glycosylated corresponding to approximately 1.8% of the molecular mass, and the glycans are known to be heterogeneous.A brief protocol for purification of SBP was worked out in our previous work. Now, a systematic evaluation of the biocatalytic properties of SBP was carried out in this study. The results shown that SBP is able to retain its catalytic activity under wide of pH and at elevated temperatures. Furthermore, SBP is very stable at high temperature, extremes of pH and in organic solvents. We also measured the apparent Michaelis constant (Km) of SBP for the oxidation of guaiacol by hydrogen peroxide.Soybean peroxidase is similar to horseradish peroxidase, a popular enzyme for generating enzyme-labeled conjugates. SBP can be used to generate enzyme conjugates with superior thermostability, pH stability and stability in organic solvents over HRP. In this study, we labeled antibody molecules with SBP other than HRP using the most common method. Then we evaluated the antibody-enzyme complex by enzyme linked immunosorbent assay (ELISA).In summary, we observed that SBP possesses substantially high catalytic efficiency and conformational stability, and remains stable over a wide pH range and at high temperature. These superior qualities of SBP may, in the future, help it to replace HRP in diagnostic, biosensor, and biotechnological uses, especially in wastewater treatment.