Microbial Strains (60) Co-gamma-ray Radiation-induced Biological Effects Of Testing And Research

Recently research shows that Huperzine A is not only a powerful selective inhibitor of acetylcholinesterase but also a helpful medicine for enhancing study and memory. Because of its great characters such as unique pharmacological profile and low toxicity have exceed other similar medicine, it has attracted widespread attention as a new generation of inhibitors of acetylcholinesterase which acts as a clinic medicine for Alzheimer's disease, AD.However, Huperzine A has a low content in its natural resources (Huperziaserrata(Thunb)Thev) and to obtain that kinds of plant also become very difficult. Because Huperziaserrata(Thunb)Thev need 8-10 years to grow up. Meanwhile, Huperzine A coming from chemical synthesis also has its innate deficiency, such as lower activity, stability and unforeseen side effects. However, fermentation is a hopeful method for producing Huperzine A if we can select the proper mycelium.In our work, we use different dose of ⁶⁰Co—γ rays (100Gy, 200Gy, 300Gy, 500Gy, 1000Gy, 2000Gy, 3000Gy)as radiation source to irradiate epiphyte. Through antagonism test and fermentation of the mycelium, we determine its growth line and metabolism line. Meanwhile, by processing the fermentation culture medium and obtain the overall alkaloids, we use the HPLC and HPLODAD-ESI-MS/MS methods to determine Huperzine A. The results show that the best mutant comes from 1000Gy of irradiation dose and 10Gy/min of dose rate. Comparing by wild epiphyte, the mutant enhances its overall mycelium and Huperzine A by 42% and 83% respectively. Our work provide a theoretical and experimental foundation for producing natural Huperzine A and this work has not been reported till now.