Using Ovule Culture Of Sugar Beet M14 Mode Of Reproduction

The regenerated plants of monosomic addition line M14 of sugar beet wasacquired from unpollinated ovules, and then genetic relationship between theregenerated plants and the parental plants was analyzed by performing AFLP, thus themode of reproduction of M14, obligate apomixis or facultative, was identified by meansof molecular biology. The experiments are as follows:First of all, in the in vitro culture of unpollinated ovules, influence of weather and materialsstorage at 4℃on contamination rate when inoculating was analized. According to the results,sampling in a fine day and inoculating on the same day was determined. Optimal medium for invitro culture of unpollinated ovules were MS+0.1mg/L NAA+1.0 mg/L 6-BA+6% sucrose,while for strong seedling and propagation of regenerated plants was MS+0.5mg/L 6-BA+3%sucrose. We found that the plant regeneration frequency of unpollinated ovules in M14 was only0.1%. The results provided materials for analyzing the genetic similarity coefficient of genomicDNA between regenerated plants and parental plants. Meanwhile, they also laid a foundation forstudies on unpollinated ovules development process and molecular mechanism.The genetic relationship between the regenerated plants with 19 chromosomes, theregenerated plants with 9 chromosomes, the progeny of apomixis with 19 chromosomes andparental plants was analyzed by AFLP, and then we confirmed that the regenerated plants with19 chromosomes were apomictic progenies, the regenerated plants with 9 chromosomes weresexual reproduction progenies. For apomixis and sexual reproduction both happened in M14,the mode of reproduction of M14 was facultative apomixis. Identifying the reproduction modeof M14 by means of Molecular biology provided theory basis for further studying on M14which was rare material of apomixis.