Intracerebroventricular Injection Of Lipopolysaccharide-induced Cortical And Hippocampal Dmt1 Upregulation And Fp1, The Cp Expression Down

Objective:To study the expression of ferroportin 1(FP1),ceruloplasmin(CP)and divalent metal transporter 1(DMT1)and their mRNA in cerebral cortex and hippocampus regulated by lateral cerebral ventricle injection of the lipopolysaccharide(LPS).To research the relationship between the proteins involved in brain iron metabolism,brain iron homeostasis and neurodegenerative diseases.Methods:(1)LPS(50ug/5ul)was injected into lateral cerebral ventricle of male wistar rats(3 months of age,n=20)to induce Acute inflammation.Rats were subgrouped to measure expression of FP1,DMTland CP and their mRNA.(2)Cerebral cortex and hippocampus was quickly freezed for RT-PCR analysis after injection of LPS for 6 hours.(3)The brains of rats were fixed in paraformaldehyde for immunohistochemistry staining after injection of LPS for 24 hours.Results:Expression of DMT1 and its mRNA was significantly higher(p＜0.01)in brain of treated with LPS than that of control rats.However,Expression of FP1 and CP and their mRNA was significantly decreased(p＜0.01).Conclusion:(1)The overexpression of DMT 1 and its mRNA was induced by LPS suggested that acute inflammation in brain may induce overexpression of DMTI.(2)Injection of LPS into lateral cerebral ventricle of the rats decrease expression of FP 1 and CP and their mRNA in cerebral cortex and hippocampus.It suggested that acute inflammation in brain may decrease the express of FP1 and CP.The changes of these proteins involved in brain iron metabolism may result in iron's overloding in brain.Overloding iron in brain may induce oxidative stress,and then promote nuerodegeneration diseases.