Effect Of Magnetic Fe₃o₄ Particles And Study Of The Cellulase Immobilized By Magnetic Gelatin On Enzymatic Hydrolysis Of Sunflower Seed Hull

While the technology of transforming lignocellulose into ethanol is studied, choosing low-cost, easily collective and processing raw materials to improve hydrolysate rate and sugar yields, is one of the most effective ways to reduce production costs. In another way, the status of enzymes in the technology of transforming cellulase into ethanol was proved beyond the shadow of a doubt. The technology of improving the cellulase activity also played an important part in the cutting off the production costs. In the paper, refering the sunflower seed hull as the raw materials, the enzymes as the object of study, the magnetic mechanisms of cellulase and cellulase immobilized by Fe₃O₄, the improvement of the cellulase activity were investigated to cut off the cellulase produxtion costs, and to explore the utilization of magnetic Fe₃O₄ particles in the enzymatic hydrolysis.The chief topic of the paper is adding magnetic Fe₃O₄ particles on cellulase in the enzymatic hydrolysis of sunflower seedhull. In the process of enzymatic hydrolysis of sunflower seed hull, the variations of cellulase activity,reducing sugar concentration, cellulose conversion, the analysis of pH and surface tension of hydrolysate were evaluated. In additon, the utilization of magnetic Fe₃O₄ particles as the carrier of cellulase Immobilized by Gelatin adsorption, was used to study the enzymatic hydrolysis quality of cellulase Immobilized by Magnetic Gelatin, to analysis the variations and expatiat on the reasons.The results indicated that after adding magnetic Fe₃O₄, the cellulase activity, reducing sugar concentration and conversion of cellulose had an increased between the 0.5 g/L and 2.0 g/L cases after 48 h. When the additive amount of magnetic Fe₃O₄ was 2 g/L, the cellulase activity at 60 h was improved significantly by 25.9%. It was found that the concentration of reducing sugar was increased from 6.950 mg/mL to 8.775 mg/mL with magnetic Fe₃O₄ 1.5 g/L. Simultaneously,compared with the blank, which the conversion of cellulose was 47.932%, the maximum cellulose conversion of samples with adding magnetic Fe₃O₄ was 60.531%. Besides, the stability of cellulase activity adding in times was better than in one time. It was approved that the cellulase Immobilized by Magnetic Gelatin adsorption had increased the maximum reducing sugar from 8.775 mg/mL to 9.162 mg/mL., which was 4.4% higher compared with the free cellulose under similar hydrolysis conditions.After adding biosurfactant, the maximum reducing sugar was 9.975 mg/mL, which was 13.68% higher than the blank especially.