| Recently, the development of nanomaterials and nanoscience is rapid. It has made breakthrough progress in new energy materials, biological medicine (medicine and biological imaging), environmental protection and other fields. The wide application of nanomaterial makes the environment exposure to people in a variety of ways, such as breathing exposure, skin exposure, oral exposure, and injection exposure. So nanomaterial security causes more attention. Therefore, exploring the interaction of nanomaterials and biological systems becomes the key to safely applied nanomaterials. Once, the nanomaterical enter into biological medium, because of the special properties, then quickly penetrate the cell membrane. It’s inevitable to meet biological macromolecules such as protein, nucleic acid. Nanomaterials and protein interaction may lead to not only change the structure and function of proteins, but also affect the protein biological activity. Therefore, it’s the prerequisite and foundation to study the interaction of nanomaterials and protein in the field of biomedical application.Studies have shown that the interaction of nanoparticle and proteins has closely relations with particle size, shape and surface chemical modification. The interaction may change structure and function of the protein, thus affecting other biological effects. The surface properties of nanoparticles, such as surface charge, hydrophobic, hydrogen bond,π-bond and steric hindrance, will influence the interaction between nanoparticles and proteins. As the papers use different nanoparticles as model and the nanoparticles may modify different surface ligands, it’s difficult to study the interaction between nanoparticles and proteins. Therefore, the studies is lack of comparability and systemic. Especially, it’s a lack of studies to surface properties continuous variation of nanoparticles effect of protein adsorption.In order to clarify the interaction between the surface properties of nanomaterials and proteins, this paper mainly makes study from these two aspects.In the first part, we used NaBH4 reduction method by Au-S key to synthesis a gold nanoparticle (GNP) arrays with continuous change of H-bond by adjusting the proportion of proper functional ligands. The nanoparticles ensure a consistent size and when the same surface properties showed a trend of continuous change, the other properties remain relatively stable. The GNP array was characterized by TEM, DLS, Zeta potential and also use HPLC-MS to quantitative surface ligand density.In the second part, we studied the interaction of five surface properties continuous variation of GNP arrys (surface charge, hydrophobic, H-bond,π-bond and steric hindrance) with Fetal Bovine Serum. We used Bradford method to quantify adsorbed protein on nanoparticles, and then use polyacrylamide gel electrophoresis (SDS-PAGE) and high performance liquid chromatography-mass spectrometry/mass spectrometry (LC/MS/MS) to studied the relationship between surface properties and different size of nanoparticles and protein adsorption quantity and adsorption types of protein.The results show that the more surface charge density of gold nanoparticles the greater proteins adsorption. Hydrophobic nanoparticles tend to absorb more protein. π-bond and steric hindrance has no obvious trend to protein adsorption. H-bond donor effects proteins adsorption more. The different size of nanoparticle protein adsorption mainly affected by the surface area and surface curvature. Small size of GNPs adsorb more protein no matter under the same quality and surface area. Different surface modification of gold nanoparticles adsorb different protein by mass spectrometry.We had a preliminary conclusion of the interaction of the surface properties of GNPs and proteins. It provides a certain help by regulating the nanomaterial surface properties to change the fate of nanomaterials in biological body. |
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