Study On Preparation And Properties Of Electrospun Polylactide-based Anti-adhesive Nonwoven Materials

Poly(lactic-co-glycolic acid)(PLGA) was biodegradable and biocompatible, and the degrade rate could be regulated by changing the molecular and composition of PLGA. Postoperative adhesion is one of the surgical unsolved problems, which directly affect the operation effect. Type of drug-loading biodegradable medical film as biological barrier membrane can effectively prevent postoperative adhesion, and its mechanism of action is to inflammation or injury from the surrounding tissue, preventing the invasion of the wound up fiber cells surrounding tissue. Its degradation time control can guarantee effective isolation until injury again epithelial change, so as to achieve the purpose of preventing adhesion formation.This paper designed a new type of electrostatic spinning nozzle device, compared with the traditional laboratory used in electrostatic spinning nozzle, the process can be a variety of material to get much shell nanoparticles drug fiber spinning, by cospinning material types, and different features to control the degradation rate of fiber shell.The article focus on the following several aspects of research:First of all, the PLGA drug-loading drug-loading blended fiber and PLGA matrix core-shell nano fiber were prepared by general electrostatic spinning method and coaxial electrostatic spinning method, respectively. PLGA was dissolved in a mixture of DCM and DMF with a volume ratio as shell layer solution. A mixture of Et OH and DMF used as the solvent for dissolving PVP and FA as core layer solution. The coaxial electrospun nanofibers were collected on an aluminum foil placed above the flat grounded metal plate. The applied voltage and the distance between the tip of the spinneret and the collector were maintained at positive voltage +19 k V, the negative voltage 2.5 k V and 15 cm, respectively. The shell flow rate was set to 1.0 m L/h, while the core flow rate was varied from 0.1 m L/h to 0.4 m L/h. All the electrospinning processes were carried out at around 25 ℃ and 50% relative humidity. As-prepared products were characterized with a FT-IR spectrometer(FT-IR; Nicolet Avatar 380), a video water-contact angle instrument(WCA; Samsung FA-CED camera), and a field-emission scanning electron microscopy(FE-SEM, HITACHI S-4800), a transmission electron microscope(FE-TEM, JEOL JEM-2100). It shows that the addition of PVP is able to enhance the porosity of the PLGA nanofibers membranes and the hydrophilic, along with the increase of the content of PVP corresponding PLGA nanofibers membranes pore first increases then decreases, water contact Angle smaller, its hydrophilicity can be improved, add a certain amount of PVP can contribute to the improvement of the hydrophilicity of PLGA matrix nanofibers membranes. The mechanical strength of PVP is worse than PLGA mechanical strength. The addition of PVP in nanofiber will reduces the PLGA nanofiber mechanical properties.Secondly, the drug-loading rate and the cumulative releases of drug from drug-loaded fibers were examined by ultraviolet spectrophotometer. It showed that the drug-loaded PLGA core-shell structure early release drugs for nanometer fiber membrane drug burst-release, mid stable release and late accelerated release three stages.Researched the behavior of drug release properties of nanofibers, the FA-loaded PLGA/FA blend nanofibers membrane has suddenly release with the other three for nanofibers, and burst release time is longer than the otherd. The model fitting of drug-loading nanofibers in vitro drug release were showed that first-order kinetics model can well describe the FA release properties.Finally, the electrospun mats of the FA/PVP/PLGA shell/core composite nanofibers membranes were dried under vacuum for 24 h at 25 ℃ before the cytocompatibility evaluation and the anti-adhesion test compared with the preparation of PLA commercial film, results show that the cells in the FA are difficult to stick PVP/PLGA composite nanofiber membranes. This further validate that FA/PVP/PLGA composite nanofibrous membrane has good anti-cell adhesion performance.