Strain Of Phenol Degrading Bacteria HY-1,identification Immobilization And Applied Research

A strain(HY-1) isolated from wastewater and sludge of Huai’an Qingjiang Pesticide Factory was cultivated, with phenol serving as its sole carbon and energy sources.Through PCR, the author amplified the16 S r RNA gene sequence of HY-1 to determine its nucleotide sequence, thus obtaining its physiological and biochemical properties. The result showed that the bacterial strain had strong capability to biodegrade phenol. According to its physiological properties as well as homological and phylogenetic analysis of 16 S r RNA gene sequence, the author identified HY-1 as Acinetobacter sp..The following factors affected the growth of bacteria and their degradability: initial concentration of phenol, temperature, p H value, liquid amount and concentration of additional salt. After 6 hours, phenol with a concentration of 500 mg/L was degraded by more than 95% under the conditions of 35°C, p H7.5, liquid amount of 50 m L,the quantity of inoculation 20%, concuss speed of shake bed 120 r/min. High concentration of additional salt had great inhibition on the growth of bacteria and their biodegradation capability with phenol. Immo-bilization improved the phonel degradation rate of bacteria. The experiment offered a comparatively reliable parameter for treating phenolic wastewater.A combination of attapulgite adsorption and species biodegradation was adopted to treat waste water with phenol. Through acid and organic modification, the surface area of attapulgite became larger and its inner aperture longer. Attapulgite had stronger hydrophilication, with more charges distributed on the surface.To know which method could achieve the best result, the author adopted attapulgite processing,by,purification, the acid-modification and,organic modif ication respedtively. The organically modified attapulgite-strain composite carrier performed the best, its degradation rate of phenol reaching 97%, followed by acid-modified attapulgite-strain composite carrier, whose degradation also reached 84%; the last was attapulgite-strain composite carrier, whose degradation was only 61%.