Fluorescent Probes For Hydrogen Peroxide, Hydrogen Sulfide And Zinc Ion

Due to its good advantages, such as easy operating, high sensitivity, good selectivity and small destruction, the technology of fluorescence imaging develops rapidly. Especially in the field of biology, chemistry and medicine, it plays an important role recently. Nowadays, more and more probe molecules are applied into the molecular field to detect small bioactive molecules and ions existing in cells, for example, ROS, RNS, RSS, enzyme and metal ions. In this paper, based on hydroxycoumarin / anthraquinone fluorophores, several fluorescent probes were synthesized and implied them to test some small molecules and ions, including hydrogen peroxide, hydrogen sulfide and zinc ion. These probes have good selectivity and high sensitivity. There are four chapters in the paper, and we will summarize them as follows:Chapter1. The Introduction, in this part the basic conceptions of the fluorescent probe and its mechanisms were mainly introduced, and the former’s works also described. Furthermore, ours design was presented.Chapter2. Based on binucleophilic reaction of hydrogen peroxide under physiological conditions for dioxygen anions, the probes FAA-MC-OH and FBA-MC-OH were synthesized, of which, the latter is better for its good selectivity and high sensitivity to H2O2.Chapter3. The probe AQ-PA was synthesized to detect Zn2+ in the condition of ethanol:water = 9:1. However, for its selectivity to Zn2+ is not ideal, the further work is in progress.Chapter4. In the last part of the paper, the probe AQ-SA-CP was synthesized, which has a good selectivity to Cu2+ in the condition of ethanol: water= 9:1, and forms a stable complex named AQ-SA-CP-Cu with the fluorescent intensity cancellation. When S2-is added into the mixture, the fluorescence recovers because S2-could replace Cu2+ from the complex for its more strong affinity to Cu2+.