Application Of Cellulase Producing Microorganisms In Agricultural Straw Resources

At the present stageat,straw production is very large in our country,but for now there is no effective way to use straw. In order to achieve sustainable agricultural development and solve the problem of resource utilization of straw.While reducing environmental pollution problems caused by the burning of straw and other improper handling caused.The first experiment were screened for the university College of Life Science and Engineering Laboratory obtained through genetic engineering project three strains of T. reesei strain producing cellulase activity were screened to determine the amount of three microbial enzyme production and growth, and ultimately concluded:Which numbered 2-5 reesei project activity lower;numbered 10-7 T. reesei engineered bacteria showed activity changed much, and unstable; numbered 8-5 reesei engineered bacteria activity during the experiment higher and more stable, more suitable for subsequent experiments. At the same time based on the measured rate of sugar, numbered 8-5 draw reesei engineered bacteria have a higher rate of straw decomposition, further validate the accuracy of the previous step of the experiment, to finalize the number 8-5 Trichoderma reesei engineered bacteria can be further used for subsequent experiments.Secondly, the magnitude of Trichoderma products were digested straw research, draw straws after T. reesei engineered bacteria degradation of polysaccharides containing no starch material.According to gas chromatography experiments and found that the polysaccharide s L-rhamnose(C6H12O5),monohydrate(C5H10O5),L-Arabinose(C5H10O5),xylose(C6H12O6),Man nose(C6H12O6),Galactoseand (C6H12O6) other monosaccharides.Polysaccharide composed of its great relationship, and study the composition of the polysaccharides are polysaccharides active follow-up study on the basis of experiments, so this experiment is the future of the straw decomposition properties of polysaccharides produced by the foundation, straw resources in favor of further exploitation.Finally, this experiment also reesei engineered bacteria produce a polysaccharide degradation of straw fermentation conditions optimized by single factor experiment, obtained in straw dosage 4%, initial pH 5.5, temperature 30 ℃, speed 200r/min, inoculation concentration 10// mL, the fermentation time of the first five days, when the liquid volume is 75mL numbered 8-5 reesei engineered bacteria can reach maximum activity. Univariate laws often can not accurately predict the interaction between various factors, so this experiment subsequent use Plackett-Burman (P-B) methods of screening an important factor affecting the level of conditions when enzyme production, obtained fiber-producing Trichoderma reesei significant influence luciferase arranged as follows:Culture temperature> incubation time> liquid volume> inoculation concentration> straw amount> speed> initial pH value.Among them, the fermentation temperature, fermentation time, was the most significant amount of impact loading, further use of Box-Behnken experimental design method to optimize the culture conditions, and finally by the response surface analysis shows that, to determine the optimum culture conditions for the culture temperature 32 ℃, fermentation time 5.5 days, liquid volume 75ml...