Toxic Effect Of Nickel Oxide Nanoparticles On Daphnia Magna

In order to investigate the toxicity of nanomaterials exposed to the water for aquatic organisms, in this paper the organism being tested is Daphnia magna the,discussing the toxic effects of nickel oxide nanoparticles on aquatic organisms.The main findings are as follows:Testing the acute toxicity of nano nickel oxide to Daphnia magna referencing to the standard method of OECD202.The LC50 (48h) Nickel oxide nanoparticles to Daphnia magna was 36.79mg/L.Investigating the reproductive toxicity of the Daphnia magna after exposing to nano-nickel oxidereferencing the OECD211 standard methods.The results showed that:long-term exposure to nano-nickel oxide (21d) leads to the time extension of parent first prolonged spawning, the reduce of spawning number and the total number of eggssignificantly.Carrying acute toxicity testto Daphnia magna through three methods:culture media exposure,environment exposureand ingestion exposure, the LC50 values were 22.21mg/L,3.79mg/L and 0.67mg/L respectively for the three modes.Discussing the oxidative damage,antioxidant enzyme activity and HSP70 expression variation amount of Daphnia magna under non-lethal dose of nano-nickel.The results showed that,nickel oxide nanoparticles cause oxidative damage to Daphnia magna,the activity of SOD,CAT and GST is increasing with the increasing concentration of nickel oxide nanoparticles,while the POD activity is decreased after the increased as the increased of stress levels.And the HSP70 expression increased exposed to Nano nickel oxide.Discussingthe markers of oxidative damage,oxidative stress and HSP70 response of in Daphnia magna with non-lethal concentrations under exposure condition.The results showed that:in the low concentration (1mg/L) of nickel oxide nanoparticles,HSP70 and GST expression increasedin the meantime to resist stress.While in the high concentration (10mg/L) the expression of HSP70,SOD,CAT and GST increased,the GSH content increased to against stress.It indicated that,HSP70 and GST are more sensitive under conditions of light pollution (lmg/L),which can be used as early warning detection of pollution indicators.