Synthesis Of Non-spherical Glycol-nanoparticles And Their Interaction With Protein And Cells

Synthetic glycopolymers with pendent sugar moieties are able to interact with lectins through multivalent ligands in a similar manner to natural glycoproteins. Nanoparticles due to their high surface/volume ratio and small size, lead to very unique properties compared with bulk-matter, such as optical, mechanical, thermodynamic properties, and NPs have shown great potential in nanomedicine and other biological applications. NPs with glycopolymers on the surface are one of the desirable bio-active particles and an important material to investigate. At present, most of the synthetic glyco-nanoparticles are spherical,research has found both by experimental and theoretical studies that particle shape has obvious effects on nanoparticle-cell interaction. In order to explore the effect of shape on glyco-nanoparticles’ biological properties and applications, two non-spherical glyco-nanoparticles with variable shape are prepared via novel synthesis method, then we study their recognition ability with lectin and cell. The main topics of our studies as follows:(1) Synthesis of non-spherical glycopolymer-coated iron oxide nanoparticlesBy combing biomimic catecholic chemistry and effect thiol-ene reaction, we are first prepared two different non-spherical glyco-nanoparticles. First, iron oxide nanoparticles with two different shapes(spindle and cubic-like) were first obtained and used as a core that was coated with dopamine methacrylamide(DMA) via catecholic chemistry for the introduction of vinyl groups. A series of molecular weight controllable glycopolymer were synthesed through reversible addition fragmentation chain transfer polymerization(RAFT), and then conjugated to the DMA-coated iron oxide nanoparticles via a thiol-ene coupling reaction. Experimental results show that, the glycopolymer-coated iron oxide nanoparticles shape control and fixed sugar density.(2) Bioactivity of non-spherical glyco-nanoparticlesIn this study, we through analysis and compare two different shape glyco-nanoparticles interacted with lectin and cell to study shape effects on nanoparticle-cell interaction. First, to investigate the adsorption property of non-spherical Glyco-IONPs, BSA, serum and Con A were used to determin whether they are stable and have specific ligand-lectin ability. Then, Hela cells were chosen as a model to test the uptake behavior of the Glyco-IONPs toward cancer cells. Experimental results show that, glyco-nanoparticles with variable shapes are stable in serum and solubility as well as enhanced activity toward specific lectins. Nanoparticle uptake in cell are depended upon shape, and cubic-like shaped particles have a higher probability of entering the cell in comparison to spindle-shaped nanostructures.