The Synthesis Of H-4edta-ph,H ₅dtpa-ph And H₆ttha-ph Ligands And Studies On Interaction Of Their Rare Earth Eu（Ⅲ） Complexes With Protein Molecules

In this paper, three novel aromatic aminopolycarboxylic acid compounds, H4edta-ph, H5dtpa-ph and H6ttha-ph were synthesized by improving experimental method. Their corresponding Eu(III) complexes, Eu-edta-ph, Eu-dtpa-ph and Eu-ttha-ph, were successfully prepared through heat-refluxing method. As a comparison with traditional aliphatic chain aminopolycarboxylic acid compound, the Eu(III) complex with H5 dtpa and H6 ttha ligands, Eu-dtpa and Eu-ttha, were also prepared by the same method. We take the Eu(III) complexes as research object combining with the target molecules of BSA and HSA to undertake experiment in this paper. Whether the Eu(III) complexes and the protein molecules exist interaction is the main purpose of this paper, which have the very important significance for understanding their metabolic process in the organism and biological effects. It is well known the construction of the biomolecule plays a crucial role in the interaction between these compounds and the biomolecule. With the rapid progress of modern science and technology, the degree of biomolecule study has been transmitted from general expressive science to probing specific chemical structure of molecular. The most basic living substance in living body is proteins and nucleic acid and the two major components play an important role in organisms and the life activities. The DNA molecular acts as the decisive role in the biological evolution process, while the protein such as BSA, HSA is the direct expression of these genetic traits. So the research of proteins and nucleic acid directly involves many contents studied in this paper. The methods of ultraviolet-visible(UV-vis), fluorescence and circular dichroism(CD) spectroscopies are utilized to study the respective interaction of Eu(III) complexes with bovine serum albumin(BSA) and Human serum albumin(HSA). This paper mainly contains three parts as following:1. In this section aromatic aminopolycarboxylic acid compound, H5dtpa-ph was synthesized. Their corresponding Eu(III) complexes, Eu-dtpa and Eu-dtpa-ph were successfully prepared. This part mainly studies the interaction mechanism between BSA and two kinds of Eu(III) complexes(Eu-dtpa and Eu-dtpa-ph). Then obtains that the two kinds of Eu(III) complexes for BSA molecular fluorescence quenching is static quenching mechanism after calculation their binding constant, binding-site number and binding distances. And that the Eu-dtpa and Eu-dtpa-ph interact with the BSA by the different way respectively, obtained by the method of circular dichroism spectrum. Furthermore, the effect of Eu-dtpa and Eu-dtpa-ph on the configuration of BSA by circular dichroism spectrum and the interaction mechanism between the europium complexes and BSA is also obtained.2. In this section aromatic aminopolycarboxylic acid compound, H6ttha-ph was synthesized. Their corresponding Eu(III) complexes, Eu-ttha and Eu-ttha-ph were successfully prepared. This part mainly studies the interaction mechanism between HSA and two kinds of Eu(III) complexes(Eu-ttha and Eu-ttha-ph). Then obtains that the two kinds of Eu(III) complexes for HSA molecular fluorescence quenching is static quenching mechanism after calculation their binding constant, binding-site number and binding distances. And that the Eu-ttha and Eu-ttha-ph interact with the HSA by the different way respectively, obtained by the method of circular dichroism spectrum. Furthermore, the effect of Eu-ttha and Eu-ttha-ph on the configuration of HSA by circular dichroism spectrum and the interaction mechanism between the europium complexes and HSA is also obtained.3. In this section aromatic aminopolycarboxylic acid compound, H4edta-ph was synthesized. Their corresponding Eu(III) complexes, Eu-edta-ph and Eu-dtpa-ph were successfully prepared. This part mainly studies the interaction mechanism between HSA and two kinds of Eu(III) complexes(Eu-edta-ph and Eu-dtpa-ph). Then obtains that the two kinds of Eu(III) complexes for HSA molecular fluorescence quenching is static quenching mechanism after calculation their binding constant, binding-site number and binding distances. And that the Eu-edta-ph and Eu-dtpa-ph interact with the HSA by the different way respectively, obtained by the method of circular dichroism spectrum. Furthermore, the effect of Eu-edta-ph and Eu-dtpa-ph on the configuration of HSA by circular dichroism spectrum and the interaction mechanism between the europium complexes and HSA is also obtained.This paper the interaction between prepared Eu(III) complexes and protein molecule in aquesou solution were studied. The paper was expected to provide some valuable information on drug research and design, and hope that these aromatic aminopolycarboxylic acid compounds acting as a drugs with the new antitumor function would be applied to the treatment of cancer in clinical practice.