Application Of Moleculary Imprinted Polymer Technique For The Analysis And Adsorption Of Environmental Pollutant Residues

Due to the complexity of environmental samples and the trace level of target analytes, the sample pretreatment process has become the bottleneck in method development and sample analysis. Traditional sample preparation technologies, such as liquid-liquid extraction and some solid phase extraction lacked enough selectivity. Molecularly imprinted polymer solid-phase microextraction (MIPSPME) technology not only present MIP’s high selectivity, but also own the advantages of SPME. MIPSPME is a highly efficient sample pretreatment technique and has been applied widespread in the field of environmental, biological and medical analysis.There is no report about the synthesis of bisphenol A molecularly imprinted polymer using chitosan as the functional monomer. There is also no ultrasonic-assisted extraction accompanying with derivatization followed by MIPSPME to extract perfluorooctane acid from food packaging. This dissertation aims to propose a sample pretreatment method based on the molecular imprinted polymer to extract perfluorooctane acid selectively, and studyies adsorption performance to perfluorooctane acid (PFOA). The specific research content are given below in detail:1. Fe3O4-CHI-MIPs of bisphenol A (BPA) was successfully synthesized and applied for the extraction and determination of bisphenol A (BPA) in water samples by coupling with high performance liquid chromatography (HPLC) using chitosan as monomer. The Fe3O4-CHI-MIP of BPA was characterized by FT-IR and VSM determination. The amount of adsorbent, pH of water sample, extraction time and desorption time, types and volume of desorption were optimized. Under the optimized conditions, the limits of detection (S/N= 3) was 1.8 ng L-1 and limits of quantitation was 5.0 ng L-1. The repeatability was investigated by evaluating the intra-day and inter-day precisions with relative standard deviations (RSDs) lower than 3.6%(n=5). The recoveries for real water samples were in the range of 88.27-104.00%. BPA was found 6.1,7.2,61.7,107.4 ng L-1 in tap water, well water, lake water and factory water, respectively.2. A study on ultrasonic-assisted extraction accompanying with derivatization followed by MIPSPME for the extraction of PFOA from food packagingc carried out. Several parameters affecting the extraction, such as sample flow rate, sample volume, eluent type, eluent volume and the eluent flow rate were investigated. Under the optimal conditions, the limits of detection (S/N= 3) was 0.6 ng g-1 and limits of quantitation was 2.0 ng g-1. The recoveries were in the range of 86.4-103.0% with a relative standard derivation less than 7.1%. This method was successfully applied for the analyzing of perfluorooctanoic acid in food packaging.3. The study on the adsorption behavior of PFOA in waste water using MIPs as an adsorbent. The factors influencing the sorption kinetics and thermodynamics of the MIP, including pH value and ionic strength were investigated in detail. The results showed that the adsorption equilibrium time was 3 h. The adsorption kinetics follows pseudo-second-order rate equation and the adsorption process conformed to Langmuir model. Under the condition of pH was 3.0 and NaC1 (W/V) was 5%, the remove rate of PFOA was 95% and the adsorption capacity was 378 mg g-1.