Preparation And Properties Research Of Porous N-HA/PVA/CF(PEEK) Composite Hydrogel

Artificial cornea is a special refractive device which is made of heterogeneous forming material to replace cloudy corneal tissue in the eyes. The current design of artificial cornea is mainly composed of porous skirt bracket and the optical center. The donor cornea is far from meeting the needs of corneal transplant, so it is of great importance to study on artificial cornea. At present, the problems of artificial cornea transplantation are mainly due to poor biocompatibility caused prolapsed and complications. The skirt bracket is the part which has direct contact with host corneal. Most artificial cornea failed because of poor biocompatibility between heterogeneous materials and host tissue. Therefore, the ideal skirt bracket has been the restricting bottleneck of artificial cornea development.Currently, most of the artificial cornea scaffold materials reported are made of PVA、n-HA、ceramics、PTFE-like FC、PTFE. What was found in the study is that although n-HA is widely used as bioactive substances implant materials in bone repair due to its ducement to calcium deposition, it tend to stimulate proliferation of film production as an artificial cornea scaffold material. As a result, the author starts to consider the application of CF(PEEK) as a constituent material to replace or partly replace n-HA, hoping to get the material with not only the advantage of n-HA, PVA and CF or PEEK, but also excellent mechanical properties, good biocompatibility, applicable to body’s physiological environment, and reduction of complications. In addition, the porous structure can improve the biocompatibility of artificial cornea scaffold material, and is good for corneal fibroblasts and new blood vessels ingrowth, increas the long-term stability of the artificial cornea.The n-HA/PVA/CF(PEEK) composite hydrogel materials were prepared by physical cross-linking and particle-induced hole method with n-HA 、PVA and CF(PEEK). Doing Study on theproperties, structure and biocompatibility of the composite hydrogel. The study also explored the preparation and application of artificial cornea in the end. The research includes the fo1 lowing aspects:1. Porous n-HA/PVA/CF composite hydrogel material was prepared by freezing-thawing method. The properties of the composite hydrogel, including the structure, the morphology, the water content and the mechanical strength were tested. The material properties and the crystal structure of PVA in the n-HA/PVA/CF composite hydrogel with different CF and n-HA were investigated by porosity water content, tensile strength, welling ratio, XRD and TG-DTA. The results show that the holes in n-HA/PVA/CF composite hydrogel were evenly distributed and interconnected. The water content of the n-HA/PVA/CF composite hydrogel is around 68% to 76%. The tensile strength is 0.6~1.2MPa. Calcium deposition test trials show that adding CF to reduce the proportion of n-HA in the scaffolds improves the situation of calcium deposition in n-HA/PVA/CF composite hydrogel.2. Porous n-HA/PVA/PEEK composite hydrogel material was prepared by freezing-thawing method. The properties of the composite hydrogel, including the structure, the morphology, the water content and the mechanical strength were tested. The material properties and the crystal structure of PVA in the n-HA/PVA/PEEK composite hydrogel with different PEEK and n-HA were investigated by porosity water content, tensile strength, welling ratio, XRD and TG-DTA. The results show that the holes in n-HA/PVA/PEEK composite hydrogel were evenly distributed and interconnected. The water content of the n-HA/PVA/PEEK hydrogel composite is around 68% to 78%. The tensile strength is 0.4~1.2MPa.3. The results of biocompatibility experiments of n-HA/PVA/CF composite hydrogel and n-HA/PVA/PEEK composite hydrogel materials showed no evidence of acute toxicity, in vitro cytotoxicity are both 0 or 1 grade, skin reaction are both very slight and delayed-type hypersensitivity are both 0 grade. This means n-HA/PVA/CF composite hydrogel and n-HA/PVA/PEEK composite hydrogel both possess good biocompatibility. They are both qualified medical material which conform to the national standard and belong to qualified medical materials.4. Exploratory research on the preparation and application of artificial cornea. First, make use of laser machining to prepare artificial cornea scaffold material. Then, crosslinking the transparent optical materials and skirts scaffolds for artificial cornea by the method of IPN crosslinking. Finally, conduct artificial cornea animal implantation experiments. The results of animal implantation experiments of artificial cornea showed that there were seldom transplantation rejection, extrusion, inflammation or aqueous leakage and the biocompatibility post implantation were good. Fibroblasts were growing in the pore of artificial cornea skirt scaffolds without front or back hyperplasia membrane. The artificial cornea position didn’t change and fitted well with the front and back lamellar. There were no change in anterior segment of eyes.