The Influence Of Heavy Metal And Antibiotics On Microorganisms’ Activity In Sequencing Biofilm Batch Reactor

Water resource is the origin of human survival and development.The shortage and pollution of water resource seriously restrict economic and social developmemt. Among various water environment polltions,the pollution caused by livestock breeding is significant,especially the pollution caused by swine wasterwater. With the expansion of scale and scope of intensive pig breeding industry, heavy metal and antibiotics are widely used. Quantities of heavy metal and antibiotics flowing into sewage treatment system together with excrement brings many difficulties for follow-up sewage treatment. The experiment aims at studying the influence of heavy metal and antibiotics on microorganism in SBBR, in ordet to lay a theorertical foundation for sewage treatment in piggery. By putting heavy metal represented by copper and zinc with different concentration and antibiotics represented by oxytetracycline and sulfamonomethoxine with different concentration in SBBR reactor, the author tries to explore the influence of heavy metal and antibiotics(both single and coalition influence) on microorganism. Following are study findings:(1) when the concentration of copper is less than 2mg/L, copper can accelerate respiration of microorganism. And copper with concentration of 2mg/L does better acceleration work than copper with concentration of 1mg/L and 0.5mg/L. when the concentration of copper is more than 4mg/L, copper can inhibit respiration of microorganism.And copper with concentration of 16mg/L has more powerful inhibiting effect than copper with concentration of 4mg/L and 8mg/L. when the concentration of zinc is less than 4mg/L, zinc can accelerate respiration of microorganism.And zinc with concentration of 4mg/L does better acceleration work than copper with concentration of 2mg/L and1mg/L. when the concentration of zinc is more than 8mg/L, zinc can inhibit respiration of microorganism.And zinc with concentration of 32mg/L has more powerful inhibiting effect than zinc with concentration of 16mg/L and 8mg/L.(2) Copper with concentration less than 8mg/L will stimulate microorganism metabolism, while coper with concentration more than 16mg/Lwill inhibit microorganism metabolism. Zinc with concentration less than 16mg/L will stimulate microorganism metabolism,while zinc with concentration more than 32mg/Lwill inhibit microorganism metabolism. That is to say, the influence of copper on microorganism metabolism is stronger than the influence of zinc on microorganism metabolism. The compounds of copper and zinc shows synergetic inhibition on microorganism respiration and metabolism.Copper with higher concentration will hide the influence of zinc on microorganism.(3) The influence of oxytetracycline with concentration between 0.1mg/L and 1.6mg/L on microorganism respiration is little.And the overall influence abides by a rule : inhibit first,recover latter. As time goes by,the influence of oxytetracycline on microorganism respiration can be negligible. The influence of sulfamonomethoxine with concentration between 0.05mg/L and 0.8mg/L on microorganism respiration follows a rule : inhibit first, accelerate latter, and recover finally. Both oxytetracycline with concentration between 0.1mg/L and 1.6mg/L and sulfamonomethoxine with concentration between 0.05mg/L and 0.8mg/L will stimulate microorganism metabolism. There will not be a serious inhibiting influence on microorganism metabolism like the influence of coper with concentration of 16mg/L and zinc with concentration of 32mg/L on microorganism metabolism.(4) when oxytetracycline and sulfamonomethoxine compound, the influence performed by them on microorganism respiration follows a rule : inhibit first, accelerate latter, and recover finally. oxytetracycline and sulfamonomethoxine activity of ETS effects are not significant. Terramycin and sulfanilamide obvious interactions between a oxygen pyrimidine.(5) Copper with concentration within the experimental conditions is more poisonous than oxytetracycline with concentration within the experimental conditions. The influence of compounds of copper and oxytetracycline on ETS activity is closely related with concentration of copper. With oxytetracycline compounding with copper, the inhibiting rate of influence of copper on ETS activity can be higher. The concentration of single copper which inhibits microorganism metabolism is 16mg/L. While, the concentration of copper which inhibits microorganism metabolism can be reduced to 8mg/L when oxytetracycline compounds with copper together.