Besed On A New Vascular Carrier To Develope An Aerobic Filamentous Bio-film Of White-rot Fungi To Treat Landfill Leachate

The white-rot fungi can secrete extracellular lignin-degrading enzymes to degrade a wide variety of recalcitrant compounds, which makes them very attractive for treating wastewater containing recalcitrant compounds. Nevertheless, there is a major problem in white-rot fungal reactors that it is difficult to achieve the continuous growth of White-rot fungi in reactor, and its application is restricted by tis failure. Our previous results showed that that aerobic filamentous bio-film in reactor have not maintained a continuous stable growth under non-sterile conditions. Therefore, a novel carrier, vessel carrier, was invented to achieve a new aerobic filamentous bio-film by immobilization to stimulate the growth of aerobic filamentous bio-film on carriers.The material of a vascular carrier is Velcro that is made of artificial fiber. It has a rough surface, which can improve the growth of immobilized mycelium. It has many pores, which are beneficial to the transportation of nutritive substance and oxygen and then to inhibitor the abscission of immobilized mycelium. In addition, vascular carrier can be used repeatedly.Solid cultures, liquid static culture and shaking culture were, in this paper, used to immobilize P. chrysosporium. The results showed that it is easy for P. chrysosporium to immobilize on the vascular carrier and the biomass of immobilized mycelium reached 1.52 g and 38.75 g using solid cultures conditions and the liquid static culture conditions, respectively. Furthermore, higher yields of immobilized biomass (57.2 g) were obtained in shaking culture. All of which suggested that vessel carrier is valuable in promoting the growth of white-rot fungi.Furthermore, the effects of vessel carrier on the manganese peroxidase (MnP) and production of P. chrysosporium were investigated in agitated Erlenmerer flasks. The results showed that MnP activity (33 U/L) and LiP activity (35 U/L) in the immobilized culture were more than that (0 U/L) in suspended culture, which suggested that vessel carrier is valuable in promoting the MnP and LiP production of white-rot fungi.Based upon these work, an aerobic filamentous bio-film of white-rot fungi developed on our new vascular carrier was used to treat landfill leachate. The results showed that during the whole treatment process, LiP (enzyme activity up to 138 U/L) were continuously producted by P.chrysosporium to degrade landfill leachate in and the pH of landfill leachate decreased from 8.9 to 6.6, the removal of the COD and ammonia nitrogen reached 68.56% and 93.71%, respectively.