Toxicological Effects And Avoidance Behavior Of Soil Heavy Metal Cadmium On Three Woodlice (crustacea:isopoda: Oniscidae)

Choosing three species of common terrestrial isopods of soilanimals: Armadillidium vulgare (Latreille,1804), Porcellionidespruinosus (Brandt,1833) and Mongoloniscus sinensis (Dollfus,1901)as experimental animals,using the natural soil of farmland,manually add one of the most extensive pollution area, the biggesthazards of heavy metals Cd, three species of common terrestrialisopods were exposed to heavy metals Cd respectively in laboratory,including three aspects of acute toxicity tests, accumulationexperiment and avoidance behavior test.(1) Acute Toxicity of Cadmium on three woodlice:the contactfilter paper test and natural soil test, acute toxicity relevant toexposure time and the concentration of heavy metals Cd. Filterpaper contact method which measured A. vulgare, M. Sinensis, P.Pruinosus48h half lethal concentration (LC50) were158.110,447.986,237.611μg/c㎡, natural soil method A. vulgare, M.Sinensis, P.pruinosus14d (LC50) were2489.403,5048.371,2616.785mg/kg. The relationship of three lethal concentration (LC50): Avulgare <P pruinosus <M sinensis, illustrating various different terrestrial isopods patience and sensitivity to the limits of heavymetals Cd.(2) Accumulation experiments show that terrestrial isopods cansurvive in a certain concentration heavy metals such as cadmium,but it significantly inhibited the growth of terrestrial isopods inheavy metal pollution, The final weight of woodlice has decreased,but different woodlice of are different accumulation ability to Cd,the bioconcentration factors of A. vulgar up to0.9602when15d-16mg/kg, the bioconcentration factors of M. sinensis reachedthe highest value8.3693when7d-48mg/kg, the maximumbioconcentration factors of P. pruinosus is5.4700when15d-48mg/kg. three species enrichment of heavy metals Cd relationship: Msinensis> P pruinosus> A. vulgare. M. sinensis has a significantenrichment of Cd; P. pruinosus of heavy metals Cd also haveenrichment. A. vulgare no accumulation of heavy metals Cd, justabsorbed.(3) Avoidance behavior tests showed three kinds of woodlice aresignificant avoidance reaction on contaminated heavy metals Cdsoil, but the effective concentration of Cd slightly different, causingThe effective concentration of Cd was evasive80%A. vulgare is32.801mg/kg,95%confidence interval25.365~42.892mg/kg;causing80%M. sinensis avoid the effective concentration of Cd is43.600mg/kg,95%confidence interval35.965~56.442mg/kg;80%P. pruinosus avoid causing the effective concentration of Cd was40.548mg/kg,95%confidence interval33.205~52.575mg/kg. therelationship of avoidance ability:A. vulgare> P pruinosus> M.Sinensis. Compared with the endpoint of the acute toxicity test LC50, terrestrial isopods behavioral tests response are more sensitive tothe endpoint of cadmium in soil.Comprehensive experimental results, screening sensitivespecies as an indicator of biological soil environment, the use ofheavy metal tolerant species enrichment and activation by thepossibility of restoration of cadmium contaminated soil is reached,so as to control the pollution of heavy metals in the soil in search ofsafety, economy technical approach.