| The strain which synthesizes magnetic nanoparticles was screened after enrichmentt culture, separation and purification taken from the water samples of dongchang lake in Liaocheng City, Shandong Province. It’s identified as Burkholderia cepacia and named as B. cepacia TZ-1 by physiological and biochemical analysis and 16S rDNA sequencing. B. cepacia TZ-1 are facultative anaerobe, it could synthesize magnetic nanoparticles only under the conditions of less than 1% oxygen partial pressure, so B. cepacia TZ-1 is originality cultured in the environment of micro-oxygen and 120 r/min shaking.B. cepacia TZ-1 is Gram-negative-bacilus whose size is about 1-2 μm×0.5-1 μm and has flagella. Colonies on the plate are about 3-5 mm, rounded, smooth, irregular, wet, yellow-green, translucent and sticky. It can be observed that there is 60 nm electron-dense crystalline particles around the cell membrane and 3-5 pieces 180nm electron-dense crystalline particles inside the bacteria.After extracted and purified by ultrasonic fragmentation, the main elements of sonication magnetic nanoparticles are consistent using TEM and SEM which include Fe, P, O. Magnetic nanoparticles ingredients are Fe3 (PO4)2·8H2O and Fe3O4 using X-ray diffraction and magnetic nanoparticles are monoclinic crystal.Magnetic nanoparticles have H2O2 peroxidase enzymes activity under the condion of H2O2.Compared with commodity HRP, magnetic nanoparticles have stronger affinity for TMB, and have stronger tolerance for H2O2 concentration, pH, temperature.Magnetic nanoparticles can catalyze and degradate dye into small molecules in the presence of H2O2.The decolorization of Methyl orange of azo dye, Mordant Red of anthraquinone dye, Fast Green FCF of fang methane dye, dye of red dye are investigated in this study. It’s indicated that magnetic nanoparticles have good degradation effect for four kinds of dyes above and stronger tolerance for H2O2 concentration, pH, temperature. |
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