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Syntheses And Properties Of Rhodamine-based Fluorescence Probe

Posted on:2016-04-28Degree:MasterType:Thesis
Country:ChinaCandidate:X D YuFull Text:PDF
GTID:2191330473460191Subject:Organic Chemistry
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Cu2+, Fe3+ and H+ play an important role in many physiological and pathological process at the cellular level. Therefore, it is important to detect the content of the subtance in the cell. In recent years, because of fluorescent probe with high sensitivity, high selectivity, low cost, convenient use, the fluorescence detection technology is widely used in cell biological. Rhodamine dyes possess many outstanding characteristics, such as stability of light, good solubility, easy to modify and so on, which make it more and more applied in biological cell imaging.In this paper, rhodamine B as a platform respectively react with hydrazine hydrate, etylenediamine, propylene amine, through condensate synthesized by four different rhodamine latctam intermediates, L1、L2、L3.From the intermediates start, synthesise a series of rhodamine cationic fluorescent probe.L1 respectively react with formaldehyde and benzene formaldehyde to obstain rhodamine Fe3+ fluorescence probe T1 and T2. Design and synthesize two rhodamine Fe3+ fluorescence probe T3、T4, which are based on mannich reaction, through L2、L3 respectively react with formaldehyde、coumarin derivatives. L1、L2、L3 directly react with 1,8-napthalene acid to obstain rhodamine pH fluorescence probe T5、T6、T7.The structures of seven probes were identified by 1HNMR,13CNMR, MS and IR. The recognition performance to object were studied by ultraviolet spectrometer and fluorescence spectra.The results show that the probes for object recognition has good recognition capability.
Keywords/Search Tags:fluorescence probe, rhodamine B, Mannich reaction, selective recognition, Cu2+, Fe3+, pH
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