The Research Of Genetic Diversity And Acute Toxicity Of Cu On Coelomactra Antiquata In Fujian

This paper mainly analyzed the kinship of Coelomactra antiquata living in different places in Fujian province, and introduced acute toxicity of Cu on C. antiquata.The author selected ITS1 gene in the nucleus amplified by PCR method, bidirectional sequenced the amplification products, and constructed the phylogenetic tree using Neighbor-joining(NJ) method, Maximum Parsimony(MP) method, UPMGA method. The result showed, ITS1 gene sequence can be used for genetic diversity analysis in species level, the pairwise distance matrix of Coelomactra antiquate living in Changle, Zhangzhou and Xiapu of Fujian province was closer, between 0.007-0.0095, genetic differetiation of three groups of internal and intergroup is not obvious, and the main source of variation came from base conversion; the pairwise distance matrix of groups living in Fujian province was further than that of groups living in Beihai, Lianyungang, Qidong and Rizhao in China, between 0.0285-0.0390, Fst significant test testified the figures were showed greatly significance(P<0.01) and represented obvious charterers of genetic differenciation.Adopted the method of acute toxicity experiment, the results indicated the 24h,48h and 96h half-lethal concentration(LC50) and safe concentration of Cu to C. antiquate was 9.754mg/L,6.603mg/L,4.839mg/L and 0.04839mg/L respectively.Adopted the method of static acute toxicity experiment, with the value of 96h LC50 of Cu on C. antiquate, author set one control group and five concentration experiment groups and the experimental time of 24h,48h,96h and 8days, and studied the influence of Cu to the antioxidant enzyme activity and lipid peroxidation in different organs and tissues of C. antiquata. In the experimental concentration range, the accumulation of Cu in the tissues of C. antiquata showed first increased and then decreased with the increase of exposed time and concentration, gill and digestive gland tissues in high concentration groups showed significant difference (P<0.05) and greatly significant difference (P<0.01), and Cu accumulation of foot muscle tissue showed no significant difference compared with control groups.In the experimental concentration range, gill, digestive gland and foot muscle tissues in low concentration groups (0.302mg/L,0.605mg/L), the activity of superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) induced first and then suppressed, the high concentration group (2.419mg/L,4.839mg/L) was directly inhibited, in the three kinds of tissues, SOD activity and GPX activity showed no significant difference of induced-inhibition effect under the low concentration stress of Cu and strong tolerance, CAT activity in high concentration groups indicated significant difference (P<0.05) and greatly significant difference (P<0.01). MDA content of the three kinds of tissues in low concentration stress groups (0.302mg/L,0.605mg/L) decreased firstly and then increased, and in high concentration groups (4.839mg/L) increased constantly. The content of MDA of the gill and digestive gland tissues in low concentration stress groups showed significant (P<0.05) and a very significant difference (P<0.01).The activity of SOD, CAT and GPX and the contend of MDA of C. antiquata showed sensitive changes exposed in polluted environmental, and can indirectly reflect the existence and toxicity of the pollutants in the marine environment. They boast the potential as a new generation of environmental pollution monitoring indicators of bioactive substances, and this paper also provides a theoretical basis for the establishment of biological biomarker system of monitoring the marine pollution.