Ionic Liquid Functional Separation Materials: Preparation And Its Performance For High Performance Liquid Chromatography

As one of the most efficient separation tecnique, high performance liquid chromatography(HPLC) was widely used in many disciplines, such as chemical, pharmaceutical analysis, food safety inspection, medical examination, environment detection, and life science, of which chromatographic stationary phase is the core part of HPLC system. The column efficiency, separation selectivity, and resolution are all decideded by the HPLC stationary phase. Therefore, development of high efficient chromatographic stationary phase is crucial for real sample analysis in many very important for the all walks of life.The full text is divided into three chapters.The first chapter: This chapter is divided into two parts. In the first part, the research significance and recent development of hydrophilic interaction chromatography were reviewed. In the second part, the research significance and recent development of mixed-mode chromatography were reviewed.The second chapter: A new imdazolium embedded hydrophilic interaction chromatographic stationary phase Sil-IEASP was prepared and further characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, and element analysis. With nucleosides and nucleic acid bases as the sample, the retention mechanism of the developed stationary phase was investigated and it indicated with hydrophilic interaction chromatography retention characteristics. The theoretical plate number could reach to 75,000 N/m. Furthermore, the developed stationary phase possessed molecular plane selectivity, outerformed the commonly used amino stationary phase.The last chapter: A new inidazolium embedded hydrophilic/reversed mixed-mode chromatographic stationary phase Sil-Im Cl was prepared and it was further characterized by Fourier transform infrared spectroscopy and thermogravimetric analysis. Since the developed stationary phase possessed hydrophilic/reversed retention mechanisms, thus, both hydrophobic and hydrophilic compounds could be simutaniously separated via a single column, outperformed the commercially avalible reversed Promosil-C8 column and GlobalsilTM APS column. Furthermore, the developed stationary phase was successfully used for the separation of complex secondary metabolites of Hansfordia sinuosae.