Construction Of Molecular Imprinted Solid Phase Extraction Chip And Research Of Analysis And Application

Several kinds of molecularly imprinted solid phase extraction(MISPE) columns were developed as a sample preparation technology in this paper. Meanwhile, combining MISPE and microfluidic chip, the chip of array MISPE columns was designed and used in the pretreatment of actual sample. A method was developed for the detection of some dyes in different food sample by molecular imprinting solid phase extraction coupling with high performance liquid chromatography with laser induced fluorescence detection(HPLC-LIF). This paper includes the following four parts:1. This section is introduction and firstly overviewed molecular imprinting technology, including the principle of molecular imprinting, preparation methods of molecular imprinting and the preparation conditions of molecular imprinting. Then, simply describes the current situation and technology of MISPE and solid-phase extraction(SPE) on chip. Finally, putting forward the thesis research purpose and meaning with the research background of food safety.2. A method for sensitive determination of safranine T in wolfberry by MISPE coupling with HPLC-LIF was developed. Using safranine T, methacrylic acid and ethylene dimethacrylate as template, function monomer, and cross-linker, respectively, the MISPE column was prepared by water-bath in situ polymerization. The morphologies of polymers were characterized by scanning electron microscope(SEM) and the properties of the MISPE capillary monolithic column including capacity and specificity were investigated under optimized conditions. The results showed that the method of recoveries were 91.2-92.9%, with RSD ranged from 3.4-4.2% and detection limit(S/N=3) of 0.4 ng/g. The capacity of column was 0.066 μg/mg and enrichment factors were higher than 90-fold.3. A method was developed to sensitively determine phloxine B in coffee bean by molecularly imprinted polymers coated graphene oxide(GO) solid-phase extraction(GO-MISPE) coupled with HPLC-LIF. The GO-MISPE column was prepared by water-bath in situ polymerization, using GO as supporting material, phloxine B, MAA, and EDMA as template, functional monomer, and cross-linker, respectively. And the properties of the homemade GO-MISPE capillary monolithic column, including capacity and specificity, were investigated under optimized conditions. The GO-MIPs were characterized by SEM and Fourier transform-infrared spectroscopy(FT-IR). The mean recoveries of phloxine B in coffee bean ranged from 89.5% to 91.4% and the intra-day and the inter-day RSD values all ranged from 3.6% to 4.7%. The detection limit(S/N=3) of 0.3 ng/g and the capacity of column was 0.040 μg/mg.4. A method for sensitive determination of rhodamine B in chili powder by the chip of array MISPE columns coupling with HPLC-LIF was developed. The chip of array MISPE columns was designed and made by PDMS substrate and glass. The capillary column was prepared by water-bath in situ polymerization, using rhodamine B, MAA and EDMA as template, function monomer and cross-linker, respectively. The properties of MISPE capillary monolithic column including capacity and specificity were investigated and the morphologies of inner polymers were characterized by SEM. Moreover, difference of MISPE columns and chips of array MISPE columns were investigated. The results showed that the method of recoveries were 83.7-88.4%, with RSD ranged from 2.5-4.0% and detection limit(S/N=3) of 0.05 ng/g. The capacity of column was 1.994 μg/mg and enrichment factors of the chip were higher than 110-fold.