Preparation, Characterization And Drug Loading Capability Of Composite Magnetic Nanoparticles

In modern medicine, as chemothe rapy has effect on killing tumor cell so be the preferred treatment for cancer after surgical treatment. However, excessive dosage brings damages to normal human cells. The multi- functional drug carrier became a popular research which can overcome over dose and reduce normal tissue damage. This study prepared three kinds of magnetic drug carriers which owned loading capacities of cisplatin, and the structural characterizations and medicine loading capacities were determined.Preparation and characterization of magnetic iron oxide nanoparticles: magnetic nanoparticles were prepared by co-precipitated chemical method. The morphology and distribution of particle size were observed as 20 nm by TEM, the kinetic diameter(Dv) was 78 nm and the theoretical particle size was 16 nm. The substance with the composition of Fe3O4 and Fe2O3.were measured by TGA. The weight percentage(wt)of Fe3O4 was 87.9 %, and wt of Ms(MNPs) was 94 emu/g. The carrier had good crystalline property and superparamagnetic property.Preparation and Characterization of CS@MNPs-COO H-CDDP: Magnetic nanoparticles were embedded by carboxylated derivative chitosan of different concentrations and grafted with cisplatin. The distribution size was about 300 nm, the mean volume particle sizes were 24 and 190 nm. Embedding and surface grafting didn`t affect the crystallinity, Ms was 43.3 emu/g, the wt of magnetic particles was 46.1 %, the paticles loaded 44.39±3.55 % cisplatin. At p H 7.4, cisplatin sustained released drug carrier half- life t1/2 was 18.7 h. IC50 values of 24 h and 48 h were 18.5 and 9.8 μg/mL, respectively.Preparation and characterization of CMCS@MNPs/CDDP: Magnetic nanoparticles were successfully embedded carboxymethylated chitosan and cisplatin. Distribution particle size was 400 nm, volume average particle diameter were 37 and 396 nm. Ms was 70.4 emu/g. The wt of magnetic particles was 74.9 %, loading 49.70±0.12 % drug. When p H=7.4, cisplatin half-life t1/2 of sustainly drug releaseing was 22.4 h. IC50 values of 24 h and 48 h were 29.5 and 10.6 μg/mL, respectively.Preparation and Characterization of SiO2@MNPs-COOH-CDDP: The distribution particle size was about 200 nm, dynamics volume average particle diameters were 58 and 220 nm. Magnetic nanoparticles has been successfully embedded in silica, and derivatized with carboxyl and grafted with cisplatin. Ms was 26.3 emu/g. The wt of magnetic particles was 27.9 %, loading 46.15±3.47 % drug. When p H =7.4, cisplatin half- life t1/2 of sustainly drug releaseing was 11.3 h. IC50 of 24 h and 48 h were 21.4 μg/mL and 10.2 μg/mL, respectively.In summary, three kinds of particles were successfully prepared and characterized by varied methods, and researches of drug functions illustrated that all the composite particles possessed their drug-loading and therapeutic capabilities.