Open-cell Foam Ceramic Immobilized Lipase And Ceramic Membrane Device Catalytic Synthesis Of Biodiesel

With the improvement of productivity levels, china’s energy consumption growing. Biodiesel resolved one of the high calorific value of renewable energy in the substance of this problem. There are much variety of materials and various methods for preparing biodiesel, such as chemical method, but pollution is serious, serious damage to the equipment. At present, more environmentally friendly and effective method is to use an enzyme transesterification.This study will first lipase fixed in silanized granular macroporous ceramic foam. Ceramic foam compared to enzyme immobilization rate before and after silylation. Nitrophenol assay using its activity, method using p-nitrophenol determine enzyme activity. After 6h fixed, measured enzyme activity than no silane enzymes high 217%. Tested the impact of the temperature, traces of water, buffer, ratio of methanol to oil, organic solvent and time change in conditions on the enzyme activity. Determine the better conditions for transformation:temperature 55 ℃, buffer oil ratio was 4.2 : 1, methanol to oil ratio of 3.5, iso –octane,in this case a higher conversion rate.Determine the stability of the immobilized enzyme experiments, such as storage stability and stability in use. Section 30 d drawn at 4℃ activity remained at about 73%,continuously for 10 times but also to maintain the vitality of about 80%.By orthogonal experiments, obtaining the immobilized enzyme experimental factors order of importance:the amount of enzyme, alcohol-oil ratio, time and temperature。Obtained from experiments optimum level factors: alcohol-oil ratio of 3.5, the amount of enzyme and substrate ratio 50 u / g, 9 hours, the temperature is 50 ℃, the actual conversion rate of 78.9%.Preparation of the annular( internal) immobilized enzyme( outer) membrane column. Method is to first produced foam ring cylindrical hole ceramic membrane;after silylation fixed ceramic membrane lipase; charged particles are ceramic enzyme in its interior; ceramic particles into enzyme the ring cylindrical ceramics inside; get catalysis and separation the core part of the enzyme membrane device. Design enzyme membrane reactor and glycerol- biodiesel separator. This enzyme membrane device, may be formed of raw materials into a continuous production of crude biodiesel product.The enzyme membrane device using response surface methodology to guide and analysis the biodiesel production process. Get good results. Obtain the optimum conditions for this device :alcohol-oil ratio 3.76 : 1, flow of 1.02 ml / s and temperature was 51.47 ℃, the actual conversion rate of approximately 87.1%. And get the conversion rate count formulas, in a certain range of conditions can be directly calculated the conversion rate from factor. Analysis shows that the conversion rate factors, order of importance are alcohol-oil ratio, flow and temperature.The enzyme membrane device and method can continuous production of biodiesel crude product from the feedstock. For the industrial production of biodiesel has some inspiration and application value.