Isolation, Identification And Whole Genome Analysis Of Bacillus Sp. LM4-2

Most of molybdenum ore resources are in the form of low-grade sulfide ore(MoS2). Using conventional smelting process, it is difficult to carry out green,economical, efficient extraction from these minerals. Therefore, the effective development of the ore in the selective leaching of valuable elements of biological metallurgy technology, because of its low cost, high efficiency and environmentally friendly features, has been extensively studied use of microbial resources and the formation of the current processing and utilization of mineral resources in the world cutting-edge technology. In this study a strain with strong resistance to Mo6+ was first isolated from Luoyang Luanchuan Molybdenum Mine soil by gradient dilution and purify. Molecular identification of the strain based on 16 S r DNA sequence and the physiological and biochemical characteristics were also studied. Furthermore, the whole genome sequencing of the strain and genome analysis was carried out. The main results are as follows:1) Sequence analysis based on 16 S r DNA showed that the homology between selected strain and Bacillus subtilis is highest, up to 95.2%. The strain was identified as a new species of the genus Bacillus, named Bacillus sp.LM4-2.2) Study on the physiological and biochemical characteristics of Bacillus sp.LM4-2 showed that the optimum growth temperature of this strain is about 30 ℃, the optimum growth pH is 6. It can grow well under the condition of 0.8mol/L Mo6+ and still grow in 1.2mol/L Mo6+. It provided a new material for the study of microbial diversity metallurgical.3) Whole-genome analysis shows that Bacillus sp.LM4-2 genome contains a circular chromosome, the total length of the resulting sequence 4,069,266 bp, and no plasmid. The genome encodes 4,153 predicted protein-coding genes. There are 116 RNA genes. In all open reading frames, there are 1411 can comment on known or predicted function. The G+C content of the genome DNA is 44.52%.4) The analysis of the metabolic pathway shows that Bacillus sp.LM4-2 genome encodes a variety of enzymes and related proteins of the citric acid cycle, glycolysis,pentose phosphate branch, oxidative phosphorylation. The genome encodes relevant genes of metabolic pathways including ribosomal transcripts and translation, cell movement, RNA processing modification, signal transduction mechanisms, cell wallsynthesis, DNA damage repair, energy production and conversion, amino acid transport and metabolism, nucleotide transport and metabolism, indicating that essential metabolic pathways of the bacteria is complete.