Study On The Nitrogen Removal Of Anammox-EGSB Reactor And The Change Of Community Structure

Anaerobic ammonia oxidation (ANAMMOX) technology is widely recognized as one of the most promising denitrification technology.It was confirmed to be a biologically mediated process which its special reaction mechanism and efficient denitrification per-formance will save the economic costs and recover the resources. The Expanded Granular Sludge Bed (EGSB) was selected as ANAMMOX reactor in this study. Nitrogen removal rate at the reactor start-up process was detected, microbial community structure of ANAM-MOX sludge and the key factor affecting the denitrification efficiency were analyzed in this study. This research provided the microcosmic scientific basis for the ANAMMOX start-up process in practical application and provided some reference to solve the problem in ANAMMOX start-up process, In addition, it provided a theoretical basis for the actual engineering application.We adopt the compound sludge to enrich the microbial community bacteria.Inoculated sludge was collected from the secondary sedimentation tank of the Harbin Wenchang wastewater treatment plant.the ratio of two kinds of sludge was threw are 1:2.In our study, In addition to use the conventional water quality,we collected inspection methods to eval-uate the running effect of EGSB reactor outside.we also collected Illumina HiSeq high-throughput sequencing analysis to analyzes the performance of different stage of start-up process and changes of microbial community of ANAMMOX sludge under control on different conditions while the start-up process was success in detail.This study verify that the EGSB reactor have dominance in start-up process of ANAM-MOX technology. ANAMMOX activity Improved about 7-fold in terms of using sludge from EGSB reactor. High-throughput sequencing showed that the ANAMMOX bacterium have existed in sludge which we enriched.During the ANAMMOX technology start-up process, Proteobacteria are always the predominant bacterium, Actinobacteria (1.93%-18.69%)> Gemmatimonadetes(033%-1.83)andPlanctomycetes(0.18%-1.61%)(belong to ANAMMOX organisms)three organisms enriched to some degree. Proteobacteriacontained some kinds of Ammonia Oxidizing Bacteria、denitrifying bacteria which floras with func-tions of denitrification The study found traces of DO ANAMMOX reactor can help im-prove some of the nitrogen removal efficiency; adding to the reactor L-cysteine on nitro-gen removal process are to enhance the role ANAMMOX.Dosing of ammonia and nitrite nitrogen removal rate has improved L-cysteine, ammonia nitrogen removal has slightly improved, not nitrite nitrogen removal effect. In ANAMMOX system adding an appro-priate amount of L-cysteine helps run ANAMMOX process.