| Microelectrode has got more and more attention in the world since it was invented. With its unique values such as tiny volume fast transport rate\ rapid response time and thin diffused field, it has been widely used by electrochemist and electroanalyst. Up to now, microelectrodes have been used in many fields such as life science, biochemistry. In this paper, we made a combined micro-electrode system which consists of carbon fibers as working electrode, a platinum wire as auxiliary electrode and an Ag wire as reference electrode. The optimum length and number of the carbon fibers used as working electrode were chosen in this paper. The principle of the Ag reference electrode and the electrochemical character of the three-electrode system were also discussed. It was found that common ions such as Nat-. NI-I4 Ca2. Mg2 and Ba2 did not interfere with the determination. We studied the voltammetric behavior of Cu ion on this microelectrode. Using this microelectrode system, we determined the copper in the blood serunr sweat~ NH4C1 and ZrCl4. Chemical Modified Electrode (CME) is the most active investigated area in current electroanalytical chemistry. We also have made a chiral amino-acid electrode for determination of D-alanine. It was made by sandwiching a newly cut slice of pig-kidney directly in between the NH3 sensing electrode and a nylon net membrane, and was activated one night in pH 8.50, 6.67x 102 mol . U?phosphate buffer (including 1 .OOx lO~ mol U?FAD). The D-amine-acid oxidase (DAAQ) in the pig-kidney could optionally reacts with only D-alanine and produced NH3 that was responded by the NH3 sensing electrode. |
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