Full Two-dimensional Gas Chromatography / Time Of Flight Mass Spectrometry For The Study Of Chinese Medicine Volatile Oils And Flavor Components In Wine

GCxGC is a novel separation method for complex samples emerging in the 1990's. After the idea was prompted and implemented, it is experiencing rapid development.In the thesis, we firstly demonstrated characteristics of GCxGC/TOFMS using petroleum samples, discussed influence of column match, temperature program, and modulation conditions on separation, and gave a strategy of system optimization.On the above basis, GC GC/TOFMS was applied to study volatile oils of Traditional Chinese Medicines (TCMs). Pogostemon cablin Benth, zedoary, Forsythia suspensa Vahl, curcuma and ginger Huang volatile oils were as samples. The chromatographic conditions of GC GC including column system, modulation time and temperature program were optimized for each sample. Total content of the components tentatively identified was more than 90 %. Pogostemon cablin Benth, zedoary volatile oils were as samples. The separation power of GCxGC and GC, and identification power of GCxGC/TOFMS and GC/MS were compared under the same conditions. The enhanced sensitivity and superior resolution of GCxGC were demonstrated. Moreover GCxGC/TOFMS provided more identification information including retention times on 1st and 2nd dimensions, similarity, reverse, probability, purity and signal/noise and so on, which make the reliability of analysis greatly increased. All of these showed that GCxGC/TOFMS is a powerful instrument in the analysis of volatile oils of TCMs.Liquor was as a sample at last, after optimizing pretreatment method and the chromatographic conditions an analytical method of liquor was established. There are 472 flavor constituents with similarity of over 800. It is documented that GCxGC/TOFMS is a powerful instrument in the analysis of complex samples again.