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Mulberry Leaves Active Ingredient Extraction And Analysis

Posted on:2005-01-17Degree:MasterType:Thesis
Country:ChinaCandidate:J ZhangFull Text:PDF
GTID:2191360125959119Subject:Special economic animal breeding
Abstract/Summary:PDF Full Text Request
This paper indicates: mulberry leaves contains many kinds of amino acid, examines out 15 kinds of amino acid, total content of amino acid which makes up the leaf protein of the mulberry is about 10% on average , among them the human body's essential content of amino acid is about 4.1% on average , accounts for about 40% of the total amount of amino acid, essential amino acid of the human body among them 7 kinds. The amino acid is widely different in different variety mulberry leaves.Content of ASP in Morus.albaL.and Morus.australis is higher than husang 32 mulberry, husang mulberry 9001 and M.atropurpurea. THR: Mulberry 9001 of lake and Morus.australis poir have the highest content. M.atropurpurea. and lake 32 mulberry and lowest. SER, mulberry 32 of lake, M.atropurpurea.are obviously lower than other varieties; The content of Pro in lake 9001 mulberry is most low, and Morus.australis poir most high. The content of MET is relatively low in several varieties, belonging to the trace.Plant albumen one restriction amino acid—content of lysine(LYS), there is about in 0.82%,it account for the total amounts the 8% about for amino acid. The glutaminic acid(GLU) is a kind of amino acid playing a role in maintaining the function while organizing in the brain, occupying the important position in the course of candy supersession and protein supersession, the content of glutaminic acid(GLU) is relatively high in the leaf of the mulberry, it is about 1% on average . Therefore, the amino acid kind is complete in the leaf of the mulberry, and the content of human essential amino-acid is relatively high, so, the protein of mulberry leave is proteins resource best in quality.The extraction technology for flavones in mulberry leaves with orthogonal test using alcohol and methanol.The result showed that as alcohol was concerned, the optimun extracting conditions were: ratio of solid to liquid as1:30, alcohol volume80%, temperture80℃ and as methanol was concerned, the optimum extracting conditions were : ratio of liquid as 1:20,methanol volume 80%,temperature 60℃Determination of flavones in mulberry leaves by capillary electrophoresis indicate that in the same producing area being different to gather issue,content of rutin and quercetin in the leaves of June are relatively low, gradual rising of July, August, September, while rutin and quercetin in the leaves of October are the highest, the content of the rutin and quercetin of November drops rapidly. In addition,different mulberry leaves of variety have too different content. For example, lake mulberry 9001 content of rutin is 2 times lake mulberry 32,and is 4 times of Morus.albaL. Morus.australis poir . and M.atropurpurea. The content of quercetin , lake mulberry 32 is the highest, the Morus.australis poir is the lowest.GABA,it is that one kind is not protein amino acid, the distribution is in extensive range, the content is relatively low in the plant body, so regard as the function factor feasibility of food not big, but it is the getting higher to be in leaf content in mulberry. For example, lake mulberry 32 is for 142 mg/100g, G M.atropurpurea .is 227 mg/100g. And the beans are only 0.04ug/g, so, eating the food or beverage with mulberry can supplement GABA . While the flavones of the mulberry is drawn, pH-value, temperature, solvent, etc. has influence to soak the appearing rate on the flavones of the mulberry. By compare it showed that extracting with alcohol is better than and with water. With the rising of temperature, the flavones macerating efficiency is improved too, but temperature drops too high instead. Density of alcohol mentioned influence relatively heavy, in 70%, 80% alcohol help the flavones get macerating efficiency , over 90% drops instead.
Keywords/Search Tags:Mulberry leaves, Protein amino acid, GABA, FlanovesExtract, Examine
PDF Full Text Request
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