| Quinolone antibiotics is the new generation broad-spectrum antibitics developed quickly and is widely used for clinic in recent year. It has the characteristic of broad-spectrum antibitics, simple structure, small side-effect and cross-resistance with other antibitics drugs. It finds widespread use in the treatment of urinary infections with a good penetration in the infected sites, so it is one of the most active fields to the exploitation of anti-infect medication. The technology of medical analysis and inspection takes an important role in medical investigation, so studied and founded the sensitive and quick analysis method is important for the new drugs exploitation, pharmacological studying and the clinic application. This thesis penetrates the sensitive fluorescence methods for quinolones, studies the effects of metal ion and quinolones and their interaction and luminescence mechanism. The main conclusion are listed below:In the first section, we summarize recent development of quinuolone synthetic antibiotics, the analytical method of quinuolone antibiotics, and the rare earths co-luminescence effect. 109 references are cited.In the second section, it is found that Al3+ can enhance the fluorescence of FLX. As studied the fluorescence characteristics of FLX, we founded that the fluorescence . was enhanced when some metal ion Al3+, Zn2+, Mg2+ were added to the system, and the enhanced effect of Al3+ was the strongest. Under optimum conditions, a linear relationship was obtained between the fluorescence intensity and the FLX concentration in the range 1.0×10-9-5.0×10-6 mol/L, and the detection limit of FLX was 7.4×10-11 mol/L (S/N=3). The method is applied for the determination of FLX in actual sample, and the result obtained is satisfactory. Comparison with common fluorescence methods for the determination of FLX, the proposed method is simple and sensitive.In the third section, the enhanced fluorescence of the NFLX, FLX and LMX system were found. When the Y3+ was added into the system, the experiments showed that Y3+can enhance the intrinsic fluorescence of NFLX, FLX, LMX system respectively. Under the optimum conditions, the fluorescence intensity is in proportionto the concentration of NFLX in the range of 1.0×10-9- 1.0×10-5mol/L, the concentration of FLX in the range of 5. 0× 10-9-1.0×10-5 mol/L respectively, and the detection limit of NFLX was 3.8×10-11 mol/L (S/N=3); FLX was 3.3 ×10-11 mol/L (S/N=3); LMX was 2.1×10-10 mol/L (S/N=3). The method is applied for the determination of NFLX, FLX in actual sample respectively, and the NFLX in mixed sample of NFLX, FLX and LMX, the result obtained are satisfactory.In the forth section, studied on the co-luminescence effect of Eu -La-NFLX-SDS systems. Using quinolone antibiotics as ligands, a fluorescence enhancement phenomenon on adding La3+ to the europium-norflorxacin (NFLX)-SDS fluorescence system was observed and studied in detail. Under optimum conditions, a linear relationship was obtained between the enhanced fluorescence intensity and the Eu3+ concentration in the range of 1.0×10-10to 5.0×l0-7mol/L. The detection limit of Eu3+ was 2.0×10-12 mol/L. This system was used for the determination of trace amounts of europium in rare earth samples with satisfactory results.The chief characteristics of this thesis are as follows:1. Found and research the fluorescence enhanced effect of Y3+- NFLX, Y3+- FLX and Y3+- LMX system, and applying it to the determination of NFLX. It explored a high sensitivity and selectivity method for detected trace NFLX, the detection limit of NFLX can fall 1-2 order by using this method. Compared with other fluorescence methods, this method is very simple, rapid and sensitive. Using standard added method, the system also applied for the determination of the NFLX in mixed sample (NFLX, FLX and LMX), the result is satisfactory .2. Studying the fluorescence enhanced effect Al-FLX system, and applying it to the determination of FLX. the mole ratio is: FLX:Al=1:10. quinolone antibiotics ca... |
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