Biomass Hydrolyzate - Levulinic Acid, Formic Acid, And Glucose Analysis And Separation Method

Levulinic acid is a highly versatile chemical with numerous industrial uses, having the potential to become a commodity chemical. It can be produced from the botanic biomass by hydrolyzing. By studying the adsorption properties of levulinic acid(LA) formic acid(FA) and glucose(glu) on six anion exchange resins, the separation process of LA was established in this paper, which was simple and reasonable.A HPLC method was devoloped for the quantitative analysis of monosaccharide, organic acids and furfural compounds. Two analytical conditions were studied: the mobile phase and the flow rate, and the optimal analytical conditions are as following: the column Shodex Sugar SH1010, the mobile phase 0.05 lg/L H2SO4 aqueous solution, the flow rate 0.5ml/min, the column temperature 60℃ and a refractive index detector.The exchange and adsorption properties of LA, FA and glu on six anion exchange resins were investigated, and the influence of the temperature was also researched, among them weakly basic ion-exchange resin 335 can provide the highest uptake capacity of LA The Freundlich equation can explain the adsorption equilibrium curves of LA on the anion resins better than the Langmuir equation. Adsorption equilibrium curves of LA on the 335 resin have been investigated at 20 ℃, 30℃ 40℃. When the temperature increased, the uptake capacity of LA decreased weakly. The velocity curves of adsorption of LA on the 335 resin at 30℃ were measured. The adsorption kinetic data fitted to the Lagergren pseudo-second order rate equation. It is demonstrated that the intrapartical diffusion was the rate controlling step.In the fix-bed ion-exchange process, after studying the six anion exchange resins with column chromatography, it was revealed that 335 resin was the properest one inseparating LA. Three column chromatographic conditions were studied: the flow rate, the elution solvent, the elution rate. The optimal condition was as following: the 335 resin in a D12*500mm column with the bed height 48.5 cm, the concentration of LA, FA and glucose was 19.5618mg/ml, 18.3879mg/ml and 17.1897 mg/ml respectively, the pH was 2.1, the flow rate was 1.09BV/h, the uptake volume was 2.55BV, the elution volume of water was 2.85BV, the elution volume of 0.6mol/l HCL was 325ml, and the yield of LA was 86.5%.