Preparation Of L-alanine Racemase And D-alanine And L-alanyl Alcohol Synthesis Process Research

L-Alanine is an important natural amino acid for man and animals. It is the most aboundant amino acid in human being's blood which has a lot to do with metabolism of sugar and has important physiological function. L-Alanine is also a supplement nutrition, it is a primary constitute portion of many composite amino acids transfusion which can also being used as medicinal intermediate, it is primary material of vitamin B6. L-Alaninol is the key intermediate to synthesize L-Ofloxacin.It can be gotten that solvent, catalyst, temperature, PH value and reacting time take a great part in the racemization of L-Alanine by studying it's racemization using carboxylic acids and base as catalyst.We used L-Alanine as starting material and got D-Alanine through Walden Inversion, and we also got D-Alanine by using DL-Alanine as starting material through enzymatic resolution. The experiment indicated that the second method was more efficient and effective.The study object in this article is to synthesize L-Alaninol by chiral pool to improve on synthetical technics and to study the factors which result in the racemization for higher yield(82.3%) and optical purity. Otherwise, two kinds of esterifying means are compared, and the conclusion is that SOCI2 is better than H2SO4.By studying the reaction mechanism and analysing the experimental result, the reaction conditinon can be gotten.