Antimicrobial Drugs And The Interaction Of Nano-drug Carriers

Recently, the research advances in drug are focused on the unity of all the excellent pharmaceutical and physiological effects, such as good solubility, stability, bioavailability and targeting delivery. In these researchs, the most advanced progress, which can make full used of pharmacological property, is the drug carriers, which based on the self-assembly system of special structure molecular or supramolecular compounds. In the paper, Triton X-100 systems, mesoporous silica materials and manganese dioxide nanoparticles were used as research media. The cephanone and the rutin, which are antibacterial, were used as research object. Particular emphasis has been placed on the increase of solubility, stability, bioavailability and targeting delivery of cephanone and of antioxidant activity of rutin. Six main experimental researches are included.1. The Effects of Cephanone on Phase Behavior and Thermodynamic Properties in Triton X-100/n-C_nH₂n+1OH /H2O SystemThe partial phase diagrams of Triton X-100/n-C_nH₂n+1OH/cephanone/H2O system were determined, and the effect of cephanone on Gibbs energy of transfer for straight-chain aliphatic alcohol from continuous water phase to Triton X-100 micelle phase was discussed. The results show that cephanone make the solubility of straight-chain aliphatic alcohol in water and that of water in straight-chain aliphatic alcohol both increase, the solubilization amount of straight-chain aliphatic alcohol in Triton X-100 micelle and that of water in Triton X-100 reverse micelle both increase, and the lamellar liquid crystal region reduce and the hexagonal liquid crystal region almost unchanged. And meanwhile, with the increase of cephanone concentration, the contribution of OH group in the alcohol to the Gibbs free energy decrease. Furthermore, in Triton X-100/n-C₈H₁7OH/H₂O system, the location of cephanone in microemulsionwas obtained, and the effects of cephanone on the stability of the lamellar liquid crystal and the structural parameters of reverse micelles composed of Triton X-100 were discussed.2. Interaction of Cephanone with Triton X-100 AggregatesThe effects of cephanone on the surface activity and aggregate behavior of Triton X-100 molecule was studied by the methods of surface tension and fluorescence, respectively. The binding constant between cephanone and Triton X-100 micelles was determined by the method of the steady state fluorescence. The location of cephanone in Triton X-100 micelles was investigated through nuclear magnetic spectroscopy(NMR). The results show that the surface activity of Triton X-100 decreased with the increase of cephanone concentration. With the increase of Triton X-100 concentration, the binding constant between cephanone and Triton X-100 micelles decreased, but the distribution coefficient of cephanone between Triton X-100 micelles and water continuous phase was invariable. The results of 'H-NMR showed that cephanone was located around the polar group of Triton X-100 micelle.3. The Effect of Triton X-100 on Electrochemical Properties of CephanoneThe electrochemical properties of cephanone and the effect of Triton X-100 on that were determined by cyclic voltammetry and A.C. impedance. The mechanism of electrochemical reaction for cephanone was deduced by coulometric electrolysis method and FT-IR spectrum. The results show that the electrode reaction of cephanone is a diffusion-controlled irreversible one, and the apparent number of electronic transfer is 0.297. The cephanone rate constant of electrode reaction decrease with the increase of Triton X-100 concentration because of the location of cephanone in Triton X-100 micelle.4. The Effect of Triton X-100 on Bovine Serum Albumin Complex with CephanoneThe interaction of cephanone with BSA and the effect of Triton X-100 on that were performed by UV-Vis adsorption spectroscopy and fluorescence spectroscopy. Theresults show that the fluorescence intensity of BSA can be quenched by cephanone because of the interaction between cephanone and BSA through the hydrophobic forces, which is mainly entropy-...