Six Multiplex Pcr Detection Of Food Pathogens

The problem of food safety is becoming more awared and more serious in many countries . It' s associated closely with the national development and people' s health and happiness. The immediate and proper treatment to patients who have eaten contaminated food is important as well as the food safty testing before food are sold or eaten by people. What' s urgent now is how to detect pathogenic bacteria in food and patients. To develop an effective and practical way to detect foodborn pathogens in this study, six species of foodborne pathogens are detected specifically and successfully in one multiplex PCR system by amplifying each target gene segment. The target genes are enterotoxin(LT) gene ( E.coli - ETEC ), invasion gene hlyA(E.coli - 0157: H 7 ) , enterotoxin gene ctxA(Vibrio cholerae), hemolysin gene (Bacillus cereus), invasive plasmid antigen gene ipaH(Shigella spp.) .The pathogenic bacteria detected are E. coli'-ETEC (Enterotoxigenic Escherichia coli), Bacillus cereus, Salmonella spp, E.coli -0157:H7, Shigella spp, Vibrio cholerae. The sensitivity of multiplex PCR system is the same with that of single PCR system at only 10. 30fg of the template. We also detect several pathogenic bacteria in 21 sorts of food and compare the detection result to that of the traditional detection method. The result of each is the same .We thus present a rapid method with both high sensitivity and specificity for the immediate detection and identification of specific pathogenic bacteria from different food materials. It can be developed into a helpful reagent kit for both clinical and food testing use.