Coli D14 ~ T, China Khiva Biodegradation Of Azo Dyes And The Humus Its Impact The Study

Azo dye was a important kind of Synthetic dyes, characterized by an or several azo-bonds (-N=N-), productions of azo dyes degradation were aroma-amines which were capable of bringing on the cancer, abnormality and mutant. Azo dyes represented about 50% on all kinds of dyes. So the research of azo dyes was regarded remarkably. The method of biodegradation was most economical and effective in the methods of dyes degradation, and the effective microorganism material for degradation was very important. Shewanella cinicaD14T was isolated from activated sludge of a textile-printing waste-water treatment plant in Guangzhou. The paper studied the biodegradation of azo dyes, the degradation route of azo dyes and degradation mechanism by Shewanella cinicaD14T. At the same time, and studied elementarily the effect of azo dyes on microbial diversity of soil by PCR-DGGE.Under the anaerobic condition, more than 95% of 1mM azo dye was degraded by Shewanella.cinicaD14T in 30 hours. The azo bound (R-N=N-R') was anaerobically reduced to amines (R-NH2, R'-NH2) of corresponding aromatic compounds by the membranene fraction only and dependent on the present of one of the electron donor such as formate, lactate, pyruvate. The aroma-amine compounds were detected by Uv-Vis spectroscopy and HPLC. These aroma-amine compounds were not degraded further under the anaerobic condition. But the double sulfonic aroma-amine compounds were degraded continuously and thoroughly when the oxygen exists, and the one sulfonic aroma-amine compounds was degraded continuously.The results suggested Shewanella cinica D14T is capable of humus respiration utilizing various organic acids and some important environmental pollutants (e.g., toluene. etc) as electron donors and AQS or AQDS as a sole terminal electron acceptor under anaerobic condition. The dissimilation reduction of 1mmol/L AQDS can couple to the production of enough ATP to support cell growth about 6 generations; The oxidization of electron donors was coupled with reduction of humus, as reduced humus increased corresponding with increasing of electron donor; The typical inhibitors such as Cu2+ which...