Ctab And Pm-19 Interaction With The Simulation Of Biofilm Electrochemical Study

In this dissertation, the progress of the research of biomembrane has been reviewed.Some fundamental properties and theories in this field are introduced. Electrochemicalmethods have been used to study different model biomembrane systems, such assupported bilayer lipid membranes (s-BLM), dodecanethiol Momolayer (DDM). Themain results are as follows:1. We have prepared l-α-phosphatidylcholine/cholesterol bilayer lipid membranes(s-BLMs) on Pt electrode. From electrochemical impedance experiments, wedemonstrated that the lipid layers on the Pt electrode were bilayer lipid membranes. Theembed of hexadecyl trimethyl amine bromide (CTAB) in the bilayer lipid membraneswas investigated using cyclic voltammetry (CV) and electrochemical impedancespectroscopy (EIS) .The experiment showed that CTAB could lead to the marker ionsFe(CN) 63-/4-crossing the s-BLMs and giving the redox reaction on the surface of Ptelectrode. Due to the reaction of Fe(CN) 63-with CTAB more intensive, it could partiallyblock Fe(CN) 64-reached the electrode surface.2. A kind of solid substrate, a Pt electrode, was selected to support lipid layermembranes. On the surface of Pt electrode, we prepared l-α-phosphatidylcholine/cholesterol bilayer lipid membranes (s-BLMs). From electrochemical impedancespectroscopy (EIS) experiments, we demonstrated that the lipid layers on the Pt electrodewere bilayer lipid membranes. The electrochemical behavior of bilayer lipid membraneson Pt electrode interacted with heteropolyoxotungstate (K7[PTi2W10O40]·6H2O, PM-19)was investigated using cyclic voltammetry (CV), electrochemical impedancespectroscopy (EIS) and atomic force microscopy (AFM). Experiments showed thatPM-19 could interact with lipid membranes and lead to the marker ions Fe(CN) 63 -/4-crossing the s-BLM. It was found that some kinds of pores had been formed on the filmby AFM. This may be helpful to understand biological activity.3. Dodecanethiol Momolayer (DDM) was prepared on gold electrode using aself-assembling procedure. Cyclic voltammetry (CV) and electrochemical impedancespectroscopy (EIS) was used to investigate the electron-transfer reaction of marker ionFe(CN)63-/4-, dopamine , ascorbic acid and hydroguinone at the thiol modified goldelectrode in the presence of CTAB. It was found that CTAB can intercalate into theself-assembled monolayer. On one hand, CTAB can improve the redox reactions ofFe(CN)63-/4-and ascorbic acid on the thiol modified electrode. On the other hand, CTABcan inhibit the redox reactions of dopamine on the thiol modified electrode. Then CTABcan slightly improve the redox reactions of hydroguinone on the thiol modified electrode.