Microbiological Culture Reactor Of Anaerobic Ammonium Oxidation Characteristics

In the wastewater treatment, with the severity of the nitrogen compounds pollution and the strictness of the environmental standard, the study and the application of the microbial nitrogen removal technology have been concerned about increasingly. Recently, a number of novel mechanisms and processes concerning nitrogen removal such as SHARON, ANAMMOX, OLAND, CANON and Shortcut Nitrification-Denitrification have appeared in the domain of wastewater treatment.In these novel processes, ANAMMOX (Anaerobic Ammonium Oxidation) process by biologically mediated has been taken for a great finding in microbiology and wastewater treatment. The discovery of the ANAMMOX process not only enriched the understandings of the nitrogen circulation in nature but also provided a new thoughtway and breakthrough possibility of the exploitation of new nitrogen removal technologies for treating ammonium-rich wastewater. In the study of this paper, it had been studied that the method of the enrichment of the ANAMMOX microorganism and the acquisition of the high activity in a plug flow immobilized floc sludge bio-reactor. The trait of the ANAMMOX process and the modal character of the microorganism were investigated in this study.The conclusion of the study are the following:1. It is feasible that the ANAMMOX population was enriched in the plug flow immobile floccule bio-reactor inoculated with ordinary nitrifying bacteria. It was possible that The ANAMMOX reactor was started up successfully and the ANAMMOX activity emerged over a period of 1.5-2 months.2. The performances of the ANAMMOX bioreactor were determined during steady state. Average influent rate was 1.194L/d (HRT=1.998d). Respectively, average influent ammonium, nitrite and total nitrogen concentration were 340mgN/L, 300mgN/L and 650mgN/L. Respectively, average influent volumetric ammonium, nitrite and total nitrogen loading were 0.1696 kgN/m~3.d, 0.1515 kgN/m~3.d and 0.3259kgN/m~3.d. Respectively, average ammonium, nitrite and total nitrogen removal rate were 99%, 98.3% and 85.9%. Respectively, average removal volumetric ammonium, nitrite and total nitrogen loading were 0.1678 kgN/m~3.d, 0.1489 kgN/m~3.d and 0.2789 kgN/m~3.d. In the entire steady operation the maximum influent and removal total nitrogen loading reached respectively 0.3457 kgN/m~3.d and 0.2814 kgN/m~3.d.3. The dissolved oxygen and influent nitrite had a great effect on the ANAMMOX reaction. The ANAMMOX process is sensitive to dissolved oxygen, but the effect was resumable and the period of the rejuvenation lessened with the increasing amount of the ANAMMOX red granular sludge. Overmuch nitrite inhibited the ANAMMOX process, but the inhibition was also resumable. The experiments showed that the inhibition appeared when the nitrite concentration was higher than 350mg/L. The nitrite concentration higher than 400mg/L caused complete inhibition of the anaerobic ammonium conversion. But the inhibition could resume to ordinary status in the period of 1-3 weeks when the nitrite concentration was declined under 350mg/L.