Chlorpromazine And Cadmium To The City To Watch The Water Body Joint Stress

As an important ataractic veterinary medicine which is widely used, chlorpromazine (CPZ) has been recognized as an emerging pollutant in aquatic environment. Under the condition of laboratory simulation, the single ecotoxicological effects of CPZ and the joint ecotoxicological effects of CPZ and cadmium (Cd) acting on Carassius auratus and daphnia magna were examined using the toxic experiment. The results of single toxic experiment showed that the LC50 value of CPZ acting on carassius auratus in 24, 48 and 96 h was 1.11, 0.43 and 0.32 mg/L, respectively, and its safe concentration for carassius auratus was 19.5μg/L. The values of 24 h LC50 and EC50 for CPZ acting on daphnia magna were 0.65 mg/L and 0.57 mg/L, and its 48 h LC50 and EC50 values were 0.36 mg/L and 0.28 mg/L, respectively. Furthermore, there were significantly positive correlations between the ecotoxicological effects of CPZ and its concentrations, and the toxicity became higher as the exposure time increased. It is a high toxicity compound for the two aquatic animals.The activities of Superoxide dismutase (SOD), Catalase (CAT) and Acetylcholinesterase (AChE) were influenced significantly when the fish were exposed to CPZ. At the same exposure time, the activities of SOD and AChE were restrained whereas the activities of CAT were induced first and inhibited then.At the same concentrations, the activities of SOD and CAT changed similarly, decreased first, then increased and decreased again, and the AChE were inhibited insignificantly. The activities of SOD and CAT in daphnia magna were influenced. The SOD activity was restained at 24 h, and induced first then inhibited at 48 h. The CAT activity was induced first and restained then at 24 h and 48 h.The LC50 value of Cd acting on carassius auratus in 24, 48 and 96 h was 19.83, 16.09 and 12.59 mg/L, respectively, and its safe concentration for carassius auratus was 3.33 mg/L. The values of 24 h LC50 and EC50 for Cd acting on daphnia magna were 0.27 mg/L and 0.19 mg/L, and its 48 h LC50 and EC50 values were 0.16 mg/L and 0.08 mg/L, respectively. Furthermore, there were significantly positive correlations between the ecotoxicological effects of Cd and its concentrations, and the toxicity became higher as the exposure time increased. It is a medium toxicity compound for carassius auratus and a high toxicity compound for daphnia magna. The joint toxicity of CPZ and Cd showed that when their toxicity was at the rate of 1:1, the joint toxicity of CPZ and Cd was antagonism on the two aquatic animals. And the antagonism effect was decreasing with time.The activities of SOD, CAT and AChE were influenced significantly when the fish were exposed to CPZ and Cd. At the same exposure time, the activities of SOD and AChE were induced whereas the activities of CAT were inhibited. At the same rate, the activity of SOD was induced whereas the CAT was inhibited and the AChE was induced insignificantly. The activities of SOD and CAT in daphnia magna were influenced. The SOD activity was restained both at 24 h and 48 h. The CAT activity was induced first and restained then at 24 h and 48 h.