Aromatic Amines In Mainstream Smoke Extract, Derived The Condition Optimization And Commercial Cigarettes Aromatic Amine Content Of The Gc / Ms Determination

Tobacco industry has been supported our government finance income. The industry focuses on the development of low harmful cigarette. Aromatic amines have been classified as carcinogens, found in tobacco smoke, in particularly 1-naphthylamine, 2- naphthylamine, 3-Aminobipheny, and 4-Aminobipheny. Therefore, it is highly desirable to establish a simple and efficient method for the trace determination of aromatic amines in tobacco smoke, to assess the safety of tobacco by the amount released of aromatic amine from tobacco smoke.It is reported that aromatic amines in tobacco smoke were derivatized as pentafluoropropionamides and measured by gas chromatography/mass spectrometry in the selected-ion-monitoring mode, which was a sensitive and accurate method. Re-examining the procedure, we found that it is not easy to get good determination of aromatic amines in tobacco smoke, due to involving many process, such as extration, derivatization, purification and control of GC-MS. Therefore, we investigated the influence of diverse parameters on the extraction and the derivatization process of aromatic amines in tobacco smoke, including the extraction mode, extraction time, extraction temperature, concentration of HCl solution, concentration of sodium hydroxide solution, pH value, derivative reagent kind , derivative temperature , derivative time, amount of derivative reagent and elution solution. Under the optimized conditions, aromatic amines could be separated and detected within 15 min. The detection limits of 3-aminobiphenyl, 4-aminobiphenyl, 1-naphthylamine, 2-naphthylamine are 0.13, 0.10, 0.12, 0.09 ng/L. The recoveries of aromatic amines ranged from 93.72 % to 104.22%. The relative standard derivations ranged from 3.21 % to 4.7 %. This method was applied to the determination of trace aromatic amines in main-stream smoke with good result. In addition, nuclear iodination has been examined preliminary, in this work as an alternative derivatization reaction. Iodination is a relatively moderate process, albeit, it is not subjected to side-reactions and not restricted to anhydrous media. After further investigation of correlation coefficient, limit of detection, relative standard deviations, and the recoveries of aromatic amines by the use of the nuclear iodination, and a promising method will be established, which is sample and efficient one for the trace determination of aromatic amines in tobacco smoke...