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Surface Modification Of Gold Nanorods And Their Biomedical Applications

Posted on:2010-11-05Degree:MasterType:Thesis
Country:ChinaCandidate:S H ChenFull Text:PDF
GTID:2191360302464596Subject:Physical chemistry
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Telomerase activity has been found in ~85-90% of all human tumors but not in adjacent normal cells. This makes telomerase a target not only for cancer diagnosis but also for the development of novel gene therapeutic agents. Antisense oligodeoxynucleotides (ASODNs) designed to hybridize with target mRNA sequences down regulate the expression of the corresponding translated proteins. Although ASODNs have emerged as an exciting and promising strategy in the field of cancer therapy in recent years, their development in viable therapeutic systems has faced challenges due to their susceptibility to enzyme degradation and poor intracellular uptake. Therefore, suitable drug delivery systems are needed to develop for effectively improving cellular uptake and stability of ASODNs. Gold nanorods,with unique properties,electronic, properties, show great promise for a range of applications in biosensing applications,biomedical imaging, and in photothermal therapy, cancer diagnostic marker, especially as nonviral vectors for DNA delivery.This paper is focused on the research of functionalized gold nanorods and its nanobiotechnology applications.1. A variety of different shapes of Au nanoparticles, such as nanorods, nanobones and nanoneedles, were prepared by seed-mediated surfactant-directed approach and controlling the different reaction synthetic conditions. Their optical properties and morphologies were characterized by UV-vis spectroscopy and transmission electronmicroscopy. The results show that the shapes of Au nanoparticles could be effectively regulated by controlling suitable synthetic parameters (e.g. the concentration , nature of Au seeds). Furthermore, the growth mechanism of various shapes of Au nanoparticles were preliminarily discussed.2. The uniform size of gold nanorods were prepared by the seed-mediated method The UV-Vis spectroscopy, transmission electron microscopy(TEM) and X-ray Diffractometer (XRD) were used to study the structural properties of gold nanorods taken from solutions water baths heated from 30℃to 90℃.Result showed that the shape of gold nanorods change from rod to spherical with increasing temperature and the longitudinal resonance band occur obversily blue shift.3. The Cytotoxicity of gold nanorods was systematically studied.. Gold nanorods are characterized by the techniques of UV-vis spectrophotometry, transmission electron microscopy(TEM),Cytotoxicity of gold nanorods is estimated from cell viabilities of SPC-A cells using the MTT assay, and the transfection of gold nanorods are studied by using EGFP-GNR conjugates.4. A novel double functionalization of carbon nanotube delivery scheme containing therapeutic gene and labeling FAM tagged ASODNs-PEI/PSS/ CTAB/GNRs. via electrostatically layer-by-layer assembling. With this novel functionalization, we have demonstrated efficient intracellular transporting, strong cell nucleus localization and high delivery efficiency of ASODNs by the PEI/PSS/CTAB/GNRs carriers. Furthermore, the ASODNs bound to PEI/PSS/CTAB /GNRs show their effective anticancer activity. Therefore, these functionalized GNRs could hold great promising for biological delivery applications and gene therapy.
Keywords/Search Tags:Gold nanorods, Thermal Control effect, GFP, Intracellular Delivery, Antisense Oligodeoxynucleotides, Gene therapy
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