Bovine Serum Albumin And Atrazine In The Gold Channel Transfer Characteristics

Nanochannel is the pores or tubles with diameter from 0.1 to 100 nanometers. It is exhibiting the great potential advantage and promising future due to the size effect and chemical physical characteristics. As an important branch of bio-nanotechnology research, nanochannels play an important role in the separation of biological materials, organic small molecules and chiral substances. The separation and detection of objectives will be greatly promoted because the characteristics of nanochannels can be significantly changed by chemical modification.Atrazine(ATR) is one of the most widely used herbicides for combating weeds in corn, sugarcane, and sorghum crops because of its selectivity against broadleaf weeds and annual grasses. Since atrazine can permeate into groundwater and rainwater easily, the large scale application of this herbicide resulted in the contamination of drinking water and soil. ATR is one kind of environmental endocrine disruptors; long-term exposure to ATR can cause breast and ovarian cancer and impact the body's endocrine and reproductive system. A number of well established analytical methods such as thin-layer chromatography, gas chromatography, high-performance liquid chromatography, and gas chromatography-mass spectrometry have been widely used for the detection and quantification of pesticides at the trace levels. Although chromatographic techniques allow high sensitivity of herbicide detection, they also require expensive equipment, organic solvents, and the prior purification of the sample. This implies a limitation on the number of samples that can be analyzed and the impossibility of using them as rapid screening methods. Bovine serum albumin (BSA) has been widely used for the preparation of immunogen of herbicides. This work focuses on the transportation characteristics of immunogen of atrazine prepared via bovine serum albumin based on nanotubule, the aspects of the work in the present thesis are shown as follows:(1) Au was deposited within the pores of the polycarbonate template membrane using an electroless deposition method. Au nanotubes membrane was successfully prepared by controling the conditions of experiments.(2) Hapten-protein was synthesized. When hapten-protein or atrazine was added into the feed cell containing antibody of atrazine, the complexes of hapten-protein/antibody or atrazine/antibody formed. The complex is larger than the free antibody and can block the current flowing through the chlorion-functioned nanochannels membrane, resulting in decreasing of current. For other herbicides, it did not decrease of current because they can not bind to the antibody. Based on the decrease of current, the method can be used for the detection of atrazine.(3) The Au-nanochannels membrane was chloridion-functionalized, the current response was studied when BSA passed through the membrane. In the electric field, a steady current is observed because electrolyte ions can pass the pores of the membrane arbitrarily. The formation of larger molecules of BSA/anti-BSA complex due to the addition of bovine serum albumin into the feed cell containing antibody of BSA can block the current flowing through the nanochannels membrane, resulting in decreasing of current. This method can be used for the determination of BSA.(4) The adsorption of BSA within the Au-nanochannels has been studied. The effects of pH, temperature, time, radius and modifier of Au-nanochannels on the adsorption of BSA in the Au-nanochannels were investigated.