Sorrel Leaves Polygonum Manganese Enrichment Characteristics Of Synchrotron Radiation Characterization

Lots of mining wasteland form after manganese mining and smelting, which pollute heavily environment. Phytoremediation attracts interest and attention from governments and enterprises as a potentially cost-effective, engineering-economical and green technique to clean up heavy metal-polluted soil. Accordingly, exploring the mechanism of manganese tolerance and hyperaccumulation is important to find new manganese hyperaccumulator plant which grows rapidly and has substantial biomass, wide distribution and broad ecological amplitude. But the hyperaccumulators is less, most of reasch still stay in stage of the wild plants screening.The purpose of this study is to expand the germplasm banks of heavy metal hyperaccumulators by screening new hyperaccumulator on the basis of investigation of Xiangtan manganese tailings wastelands. It has important theoretical significance and application value for the metal ecological restoration and pollution control of soil.The native vegetation and plants in Xiangtan manganese mining area were investigated. It was found that Polygonum lapathifolium L has super manganese patience.Under the condition of different manganese supplying, even the manganese concent is 1OOOOμmol·L-1, Polygonum lapathifolium L can grow normally. This implies that Polygonum lapathifolium L have strong manganese patience. The distribution of manganese among roots, stems and leaves in Polygonum lapathifolium L is different. The ICP-OES analysis reveals that the levels of Mn in roots, stems and leaves of the plants increase while the content of Mn2+ increasing in the culture solution. The results show that Mn distribution sequence is: leaves>roots>stems. And most of Mn is transferred and accumulated in leaves. When the Mn content of culture solution is 5000μmol·L-1,10000μmol·L-1, the Mn content of leaves reaches 18709mg·kg-1,27663mg·kg-1 respectively. There is no nutrient element deficiency. This may be Polygonum lapathifolium L's feedback mechanism at high manganese contents.Leaves FTIR curves indicates there is only methyl groups'characteristic absorption peaks(1380cm-1) in various cell membrane and wall have changes:peak height increase at first, and then decrease with the increase of concentration of manganese processing, Simultaneously, the peak also slightly moves toward the shorter wavelength. This shows the cell wall increases cation exchange absorbs even more Mn through decreasing its methylation levels, but the high content of Mn has stressed on Polygonum lapathifolium L, and then methylation began to rise.Synchrotron radiation XAFS spectra show that manganese treatment could change its form in the leaf of Polygonum lapathifolium L. In contrast condition, the manganese in the leaves of Polygonum lapathifolium L is mainly in the form of manganese, citric acid and tathion. In low manganese processing conditions, manganese in the leaf of Polygonum lapathifolium L is mainly in the form of malic acid manganes, elemental manganese and glutathione manganese. In high manganese processing conditions, manganese in the leaf of Polygonum lapathifolium is mainly in the form of malic acid.Different manganese processing can affect the distribution of manganese and other elements. Synchrotron radiation hard X-ray spectra showed that the distribution of manganese in the leaf is relatively homogeneous in the control condition. With increasing manganese content, manganese in the leaf of Polygonum lapathifolium L tends to accumulate in veins, and the distribution of other elements in leaves is similar to manganese.