Lysine Copper / Zinc Synthesis, Characterization And The Nature Of The Khumbu Acid

L-lysine, the first limiting amino acid, is a kind of essential amino acid which can't be synthesized by the body. L-lysine plays an important role in human physical activities,such as the maintenance if acid-base balance of human body, the synthesis of nucleoprotein and the promotion of regeneration of the brain cells. In addition, lysine can react with trace metal elements to synthesize the lysinate chelate compounds with ring structure. As the unique structure and properties, the chelates have become the new type nutritional additives which can supply the essential trace elements and amino acid at the same time. Moreover, lysine is the major raw synthesis material of the marine antihypertensive drug-laminine.Chelate of lysine with trace elements (copper lysinate, zinc lysinate) which has high purity and yield was synthesized using L-lysine monohydrochloride and metal hydroxides as raw materials. Orthogonal experiments were used to study the optimization of synthetic conditions. After purification, the composition and properties of the complex were characterized by a series of analysis methods, such as element analysis, infrared spectrum, ultraviolet spectrum and thermogravimetric analysis.The calculating program was designed by combining Achar differential and Coats-Redfern integral method fitting the thirty kinetic equations. The kinetic equation of thermal decomposition for chelates, the corresponding kinetic parameters, activation entropy△S≠and activation free-energy△G≠were obtained.According to the colorimetry principle of a-amino acid and ninhydrin, the method of determination of the concentration of lysine was investigated and the impacts were discussed respectively to find the optimum conditions of determination of lysine by ninhydrin colorimetry. As the 732 cation exchange resin was the main form of separation and purification of laminine, the effects of temperature, pH value and the concentration of NaCl or NH4Cl on the absorption of laminine on 732 cation exchange resin were investigated through static experiments, and the adsorption isotherm at 25℃was measured.