Synthesis Of Yvo₄: Eu Fluorescent Nanoparticles And Its Application In Biological Analysis

In recent years, the achievements about the study of nanoparticles used for markers in biological area are noticeable. The technology of nanoparticles marker in biological area had shown good prospects in the areas of diagnosis of major diseases of mankind,biochip technology,cellular and biological imaging,the rapid detection of strong pathogenic pathogen and early warning,and anti-terrorism of the biological area. Rare earth luminescent markers have the advantages of large Stokes shift,high fluorescence emission intensity, narrow half emission peaks and long fluorescence lifetime. Using of these special nanoparticles as biological analytical markers can improve the detection sensitivity of biological molecules, so they have a broad prospect in application.Here, the YVO4:Eu fluorescent nanoparticles were synthesized by a water bath. The effect of experimental conditions, such as the reaction temperature and reaction time, the pH values and molar ratio of the reactants, the percentage of europium ion doping concentrations and the best concentration of YVO4:Eu nanoparticles solution, on fluorescent characters of YVO4:Eu nanoparticles were studied. The prepared YVO4:Eu fluorescent nanoparticles were charactered by XRD, TEM, particle size distribution and IR. Finally, the prepared YVO4:Eu fluorescent nanoparticles were used to label bovine serum albumin and avidin by electrostatic interaction.The results showed that the best conditions to synthesize YVO4:Eu nanoparticles were as follows:The reactants reacted at 90℃for 120 minutes. The pH values of rare earth ions,sodium citrate and sodium metavanadate solution were 1.0,6.5 and 12.7 respectively. The results of XRD,TEM and particle size distribution showed that the average diameter of YVO4:Eu nanoparticles was below 40 nm, with good crystallization and monodispersion.The YVO4:Eu nanoparticles can couple with BSA by electrostatic interaction, and the margins of fluorescence intensity between YVO4:Eu-BSA and YVO4:Eu solution were proportional to the concentration of BSA which was in range of 0.2 to 2.0μg·mL-1. The linear equation was Y= 56.96C+26.77, with the correlation coefficient of 0.9985. In the phosphate-buffered solution of pH 7.4, the YVO4:Eu nanoparticles can label avidin by electrostatic interaction successfully. Moreover, avidin still kept their activity that they reacted with D-biotin specifically. The experimental results showed that the synthesized YVO4:Eu nanoparticles can be used as a common fluorescent markers for the detection of biological molecules.