Genetic Diversity Of Cytoplasm Heredity In Pyrus Some Main Cultivars

There were many species of Pyrus. Because many interspecific hybrids were fertile,there were so many questions on Pyrus relationships for a long time.This experiment researched on the genetic diversity of nineteen cultivars in Pyrus derived from cpDNA PCR-RFLP.Ten universal primer pairs of chloroplast genome were used to amplify cpDNA non-coding regions in nineteen Pyrus species. The PCR products were digested by seven restriction enzymes. To clone and sequence the cpDNA non-coding regions in nineteen Pyrus species. DPS v7.05 was used to account Jaccard, the dendrogram was constructed by UPGMA. DNAMAN,DNAStar,ClustalX-1.83 and PHYLIP-3.68 were used to build the system tree.According to the sequences,we could make sure the relationship among these Pyrus.The result showed that seven primer pairs(cp01, cp02, cp03, cp 04, cp06, cp09, cp 10)generated special bands from Pyrus. The combination have 931 between primer pairs and restriction enzymes.The fragements digested of cp09/MvaI, cp03/Hin6I were different.The trnS-trnfM region in chloroplast genome were cloned and sequenced in nineteen species of Pyrus. The nucleotide sequences of Korla pear and Yali were longest(1642bp),and the shorest sequences(1272bp) were Pingguoli,Zaosuli,Cili,Xiangya,Cuifu.The nucleotide sequences were aligned with DNAMAN software. The identity of the Korla pear and P.bretschneideri Rhed. was 85.01%, the identity of the Korla pear and P.sinkiangensis Yu was 78.60%, the identity of the Korla pear and P.communis L.was 78.28%, the identity of the Korla pear and P.pyrifolia (Burm.) Nakai was 77.47%, the identity of the Korla pear and Pyrus ussuriensis Maxim. was 77.91%.According to the aligment, the trnS-trnfM region in chloroplast genome were built in neigorbor way of the PHYLIP-3.68 software.According the phylogenetic tree,Heisuanli and Jingbaili were group,Fuqie and Shenbuzhi were group,Dongba and Xinshiji were group, the Korla pear,Jujuli,Jinchuanxueli, Heisuanli and Jingbaili were group.The conclusions were as follows:the average distance coefficient of the Korla pear with Yali,Dangshanli,Pingguoli,Cili,Jinchuanxueli,Jinfeng and Jujuli were smaller; that of Korla pear with the other cultivars were bigger. the Korla pear and P.sinkiangensis Yii had nearer relationships. the Korla pear and P.pyrifolia (Burm.) Nakai had farer relationships.Zaosu was hybridized by Pingguoli and Shenbuzhi. The identity of the Zaosu and Pingguoli was 99.45%, The identity of the Zaosu and Shenbuzhi was 97.76%. Jinfeng was hybridized by Pingguoli and Cili. The identity of the Jinfeng and Pingguoli was 78.76%, The identity of the Jinfeng and Cili was 78.71%.Contrasted to the male parent,the offsprings were closed with the female parent. The identity of the Korla pear and the Dangshanli were 98.36%,which were hingest in the another Pyrus species.Hence, the Korla pear was closed with P.bretschneideri Rhed.and P.sinkiangensis Yu.Therefore,the meaning of the paper was able to analyse the relationships with biology and bioinformatics.We were able to study the polymorphism in Pyrus.The evidents were provided to research the relationships of the Pyrus.